FB2024_03 , released June 25, 2024
Recombinant Construct: P{roX1-EGFP.DHS}
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General Information
Symbol
P{roX1-EGFP.DHS}
FlyBase ID
FBtp0019226
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
ProX1-DHS
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
Component allele Avic\GFPE.roX1.DHS
Transgenic product class
Component
Use(s)
Encoded product / tool
Description

A 750bp EcoRI fragment containing the lncRNA:roX1 5' region (which includes the start sites of 3 distinct 5' ends of lncRNA:roX1 RNA) is fused upstream of a promoterless EGFP reporter. The construct is not expected to produce EGFP protein, as any transcript produced is predicted to contain nonproductive ATG codons from the lncRNA:roX1 5' region. In addition, sequences encoding the DNaseI hypersensitive site (DHS) from the lncRNA:roX1 transcription unit are fused downstream of the SV40 polyA site of the EGFP reporter.

Component allele gypsy\su(Hw)BR430
Transgenic product class
Component
Use(s)
Encoded product / tool
Description

Contains 12 su(Hw)-binding sites, corresponding to bp 647-1077 in the gypsy transposon.

Construct: Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites.

Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites, nucleotides 647 to 1077 of FBrf0045014.

Consists of a 430bp gypsy\su(Hw)BR, gypsy sequences 647-1077 (coordinates of FBrf0045014).

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{roX1-EGFP.DHS}
Secondary FlyBase IDs
    References (3)