Transgenic construct that contains a Ecol\lacZarm.PV reporter flanked on each side by an insulator (scs' and scs respectively) to mitigate any chromosome position effects. A 1.1kb NotI/PspOMI fragment containing lncRNA:roX2 cDNA sequences has been inserted (upstream of the arm promoter of the lacZ reporter) to test whether the fragment has a role in dosage compensation. The lncRNA:roX2 fragment is in 3'-5' orientation relative to the Ecol\lacZ coding region.
Construct: Fragment containing scs' sequences.
Construct: Fragment containing scs sequences.