Transgenic construct that contains a Ecol\lacZarm.PV reporter flanked on each side by an insulator (scs' and scs respectively) to mitigate any chromosome position effects. A 3.7kb NotI fragment containing lncRNA:roX1 cDNA sequences followed by a 1.1kb NotI/PspOMI fragment containing lncRNA:roX2 cDNA sequences has been inserted (upstream of the arm promoter of the lacZ reporter) to test whether the fragments have a role in dosage compensation.
Construct: Fragment containing scs sequences.
Construct: Fragment containing scs' sequences.