Transgenic construct that contains a Ecol\lacZarm.PV reporter flanked on each side by an insulator (scs' and scs respectively) to mitigate any chromosome position effects. A 4.0kb NotI genomic fragment containing lncRNA:roX2 (lncRNA:roX2t4.0) has been inserted (upstream of the arm promoter of the lacZ reporter) to test whether the fragment has a role in dosage compensation. The lncRNA:roX2 fragment is in 3'-5' orientation relative to the lacZ coding region.
Construct: Fragment containing scs sequences.
Construct: Contains a 4kb genomic NotI fragment. The 5' end of nod is also included.
Construct: Fragment containing scs' sequences.