FB2024_03 , released June 25, 2024
Recombinant Construct: P{Gr28b.e-GAL4.4.245}
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General Information
Symbol
P{Gr28b.e-GAL4.4.245}
FlyBase ID
FBtp0014672
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4Gr28b.e.4.245
Also Known As
Gr28b.e-GAL4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL4Gr28b.e.4.245
Transgenic product class
Component
Use(s)
Regulatory region(s)
Gr28b
Gr28b_Gr28A3
Encoded product / tool
GAL4
Description

4.245kb of Gr28b promoter sequences regulate expression of a GAL4 driver.

(Scott et al., 2001)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
terminal organ
(Scott et al., 2001)
embryonic/larval gut | restricted
(Scott et al., 2001)
embryonic/larval mouth | restricted
(Scott et al., 2001)
terminal organ | restricted
(Thorne and Amrein, 2008)
third instar larval stage
gustatory receptor neuron of terminal organ
(Kwon et al., 2011)
larval gnathal ganglion
(Kwon et al., 2011)
gustatory receptor neuron of terminal organ | subset
(Mishra et al., 2018)
embryonic/larval brain | restricted
(Mishra et al., 2018)
larval ventral nerve cord | restricted
(Mishra et al., 2018)
embryonic/larval proventriculus | restricted
(Mishra et al., 2018)
embryonic/larval midgut | restricted
(Mishra et al., 2018)
adult stage
high salt/bitter-sensing neuron | subset of prothoracic tarsal segment 5
(Ling et al., 2014)
chemosensory neuron of labellum
(Thorne et al., 2004)
chemosensory neuron of ventral cibarial sense organ
(Thorne et al., 2004)
chemosensory neuron of prothoracic leg
(Thorne et al., 2004)
labellum
(Scott et al., 2001)
cibarial sense organ
(Scott et al., 2001)
gustatory receptor neuron | subset
(Thorne and Amrein, 2008)
gustatory receptor neuron of labellar taste bristle | subset
(Thorne and Amrein, 2008)
gustatory receptor neuron | subset of ventral cibarial sense organ
(Thorne and Amrein, 2008)
gustatory receptor neuron | faint | subset of prothoracic tarsal segment 5
(Thorne and Amrein, 2008)
gnathal ganglion | restricted
(Thorne and Amrein, 2008)
gustatory receptor neuron | subset of short labellar taste bristle | subset
(Weiss et al., 2011)
gustatory receptor neuron | subset of intermediate labellar taste bristle | subset
(Weiss et al., 2011)
high salt/bitter-sensing neuron of adult labellum sensillum
(Weiss et al., 2011)
intermediate labellar taste bristle

Comment: isoform Gr28b-RE

(Weiss et al., 2011, Weiss et al., 2011)
intermediate labellar taste bristle I-b

Comment: isoform Gr28b-RE

(Weiss et al., 2011, Weiss et al., 2011)
short labellar taste bristle S-b

Comment: isoform Gr28b-RE

(Weiss et al., 2011, Weiss et al., 2011)
short labellar taste bristle S-a | intense

Comment: isoform Gr28b-RE

(Weiss et al., 2011)
intermediate labellar taste bristle I-b | intense

Comment: isoform Gr28b-RE

(Weiss et al., 2011)
short labellar taste bristle S-a

Comment: isoform Gr28b-RE

(Weiss et al., 2011)
Additional Information
Statement
Reference

ScerGAL4Gr28b.e.4.245 drives expression of Gr28b isoform e.

(Ling et al., 2014)

ScerGAL4Gr28b.e.4.245 is expressed in gustatory neurons of all of the bitter-sensitive small labellar taste bristles, and in gustatory neurons of the four posterior-most intermediate labellar taste bristles.

(Weiss et al., 2011)

ScerGAL4Gr28b.e.4.245 expresses the isoform Gr28b-RE which is found in all of the short and in a few of the intermediate bitter-sensitive sensilla of the labellum.

(Weiss et al., 2011)

ScerGAL4Gr28b.e.4.245 is expressed in all of the short and in a few of the intermediate bitter-sensitive sensilla of the labellum.

(Weiss et al., 2011)

ScerGAL4Gr28b.e.4.245 drives expression in one gustatory receptor neuron of the larval terminal organ. This neuron also expresses ScerGAL4Gr33a.4.7, ScerGAL4Gr66a.1.8, ScerGAL4Gr32a.3.7.PW, ScerGAL4Gr39a.a.3.4, ScerGAL4Gr39a.b.1, ScerGAL4Gr59d.4.1, ScerGAL4Gr9a.6.3, ScerGAL4Gr22e.2.5, ScerGAL4Gr22a.4.2, ScerGAL4Gr28b.a.3, ScerGAL4Gr36b.0.9, ScerGAL4Gr36c.2.5, ScerGAL4Gr58b.1, ScerGAL4Gr59a.1.5, ScerGAL4Gr59c.5.5 and ScerGAL4Gr93b.5.3.

(Kwon et al., 2011)

Expression of ScerGAL4Gr28b.4.245 is seen in approximately 13 neurons per labial palp. ScerGAL4Gr28b.4.245 is found to be expressed in a single neuron associated with the ventral cibarial sense organ. Expression of ScerGAL4Gr28b.4.245 is found in two neurons associated with the fifth tarsal segment of the adult foreleg.

(Thorne and Amrein, 2008)

ScerGAL4Gr28b.4.245 expresses in 8-10 neurons innervating small or medium bristles on the labellum.

(Thorne et al., 2004)
 
Marker for
Reflects expression of
Gr28b
(Ling et al., 2014, Kwon et al., 2011, Weiss et al., 2011, Weiss et al., 2011, Thorne and Amrein, 2008, Thorne et al., 2004, Scott et al., 2001)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
57621
w*; P{Gr28b.e-GAL4.4.245}2; Dr1/TM3, Sb1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (20)