Transgenic construct used in an enhancer-blocking assay to study the function of the dwg protein and its interactions with the scs chromatin domain boundary element. A fragment containing four copies of the BSwt oligonucleotide from region C of the scs insulator element has been inserted into P{wRW}, upstream of the w enhancer and the w transcription unit. Three copies of the BSwt are in forward orientation, the 3' most one is in reverse orientation.
Construct: 0.5kb of scs' sequences.
Construct: Included in a fragment containing four copies of the BSwt oligonucleotide from region C of the scs insulator element.