FB2024_03 , released June 25, 2024
Recombinant Construct: P{HZCaSpeR}
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General Information
Symbol
P{HZCaSpeR}
FlyBase ID
FBtp0011319
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
lacZ reporter
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
Component allele Ecol\lacZHsp70Bb.HZ50
Transgenic product class
Component
Use(s)
Encoded product / tool
Description

Construct: Hsp70 promoter sequences from position -50 to +271 (including the first 7 amino acids) are fused in frame to Ecol\lacZ, promoter sequences lack all of the heat shock elements shown to be required for heat inducibility.

Hsp70 promoter sequences drive Ecol\lacZ expression.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference

Of 22 independent transformed lines analyzed, most showed little, if any, β-gal expression. However, some lines did show exhibit various levels of expression, the pattern varying from line to line. Such expression was usually observed in late embyogenesis, however some lines showed expression along the ventral midline at germ-band extension.

 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{HZCaSpeR}
Secondary FlyBase IDs
    References (2)