Transgenic construct used in a study to test the ability of insulator or boundary elements to block repression of a target gene by a Polycomb response element (PRE). A 661bp NdeI-PstI fragment containing the core activity of the major Ubx Polycomb response element (PRE, located approximately 24kb upstream of the Ubx promoter) and a 454bp EcoRI-BamHI fragment containing the core scs' element are fused upstream of the w+mC gene. The sequences are in the order PRE - scs' - w+mC.
Construct: 661bp NdeI-PstI Ubx PRE (Polycomb response element) core fragment.
Construct: 454bp EcoRI-BamHI fragment containing the core scs' element.