Transgenic construct used in an enhancer-blocking assay. Contains a ftz-lacZ reporter that includes two ftz enhancers (the UPS and NE elements) fused upstream of an Hsp70 promoter (starting at -194 and including the HSE regulatory element) followed by the lacZ open reading frame. A 1.7kb Fab-7 element has been inserted in between the NE element and the Hsp70 promoter. A w+mC minigene is present as a marker.
Construct: 0.5kb of scs' sequences.
Construct: 1.7kb NcoI-ApaI fragment containing major nuclease hypersensitive sites HS1 and HS2 plus the minor hypersensitive site.