A w+* minigene in which two amino acid substitutions (G135L and K136Q) have been introduced into the putative nucleotide binding fold of w.
Construct: 0.5kb of scs' sequences.
Full length 1.8kb BamHI-BglII scs insulator.
Construct: 1.8kb of scs sequences.
Full length 1.8kb scs insulator.
1.8kb BamHI-BglII fragment containing scs sequences.