Transgenic construct used in an enhancer-blocking assay to analyze the Fab-7 element of the bithorax complex and its ability to form a chromatin domain boundary. Derived from P{wRW+we}; a 1.7kb truncated Fab-7 element (NcoI-ApaI fragment) has been inserted between the w enhancer and w transcription unit.
Construct: 1.7kb NcoI-ApaI fragment containing major nuclease hypersensitive sites HS1 and HS2 plus the minor hypersensitive site.
Construct: 0.5kb of scs' sequences.