FB2024_03 , released June 25, 2024
Recombinant Construct: P{1.6zen-lacZ}
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General Information
Symbol
P{1.6zen-lacZ}
FlyBase ID
FBtp0004693
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZzen.1.6
Size
Expression Data
lacZ reporter
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
ry+t7.2
w+mC
Component allele Ecol\lacZzen.1.6
Transgenic product class
Component
Use(s)
Regulatory region(s)
zen_1.6
Encoded product / tool
lacZ
Description

1.6kb of zen promoter region drives the expression of a lacZ reporter.

(Doyle et al., 1989)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4 -- 6
primordial germ cell
(Doyle et al., 1989)
embryonic cycle 12 -- embryonic cycle 13
organism | dorsal

Comment: includes anterior and posterior pole

(Doyle et al., 1989)
embryonic stage 5 -- 6
organism | dorsal

Comment: also small amount of ventral expression

(Doyle et al., 1989)
embryonic cycle 14 -- extended germ band stage
(Doyle et al., 1989)
embryonic stage 12
amnioserosa
(Doyle et al., 1989)
mesoderm
(Doyle et al., 1989)
Additional Information
Statement
Reference

The 1.6 kb zen upstream region drives EcollacZ expression in a "dorsal-on/ventral-off" pattern resembling wild type zen expression. Reporter expression is initiated and maintained correctly in the dorsal region, but no signal is detected in pole cells. When the construct contains the ry marker, low level of signal is seen in ventral tissues as a result of a mesoderm enhancer element in ry; no signal is seen when w is used as the marker gene.

(Doyle et al., 1989)
 
Marker for
Reflects expression of
zen
(Doyle et al., 1989)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{1.6zen-lacZ}
(FlyBase, 1996-)
zen-lacZ (1.6kb)
(Farge, 2003)
Secondary FlyBase IDs
    References (6)