FB2024_03 , released June 25, 2024
Recombinant Construct: P{4xCME-lacZ}
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General Information
Symbol
P{4xCME-lacZ}
FlyBase ID
FBtp0004478
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZTl.4x
Size
Expression Data
lacZ reporter
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Ecol\lacZTl.4x
Transgenic product class
Component
Use(s)
Regulatory region(s)
Tl_CME
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Description

lacZ expression from a weak P element promoter governed by four copies of the ACGTG motif from the Tl promoter region.

(Wharton et al., 1994)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 9 -- 13
embryonic ventral midline neuroblast
(Wharton et al., 1994)
ventral nerve cord primordium
(Wharton et al., 1994)
midline primordium
(Wharton et al., 1994)
embryonic stage 10
larval midline neuron | subset
(Wharton et al., 1994)
embryonic stage 11
ventral midline of embryo
(Sonnenfeld et al., 1997)
embryonic stage 11 -- 15
presumptive embryonic/larval tracheal system
(Sonnenfeld et al., 1997)
salivary gland duct anlage
(Sonnenfeld et al., 1997)
late embryonic stage
midline glial cell
(Wharton et al., 1994)
Additional Information
Statement
Reference

In embryos, stage 11: expressed in CNS midline cells and cells surrounding the tracheal pits; stage 15: expressed in tracheal cells, including the anterior and posterior spiracles, in CNS midline cells, and in salivary ducts.

(Sonnenfeld et al., 1997)

In embryos, expressed in CNS midline cells beginning at stage 9; later becomes restricted to midline glia and a subset of ventral neurons.

(Wharton et al., 1994)
 
Marker for
Reflects expression of
Tl
(Wharton et al., 1994)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{C4PLZ}
(Wharton et al., 1994)
descendant(s)
Comments

report comment: Carries 4 copies of the Toll site 4 CME (Central Midline Element) driving Ecol\lacZ.

(Wharton et al., 1994)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{4X950Tl4}
(FlyBase, 1996-)
P{4xCME-lacZ}
(FlyBase, 1996-)
P{w+; 4xCME-lacZ}
(Estes et al., 2001)
Secondary FlyBase IDs
    References (6)