FB2024_03 , released June 25, 2024
Recombinant Construct: M{MlwB}
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General Information
Symbol
M{MlwB}
FlyBase ID
FBtp0001682
Feature type
transgenic_transposable_element
Component Allele(s)
Dmau\mariner\TpA2L
Ecol\lacZP\T.A92a
Size
Expression Data
lacZ reporter
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

M{MlwB} is an enhancer trap construct that contains at its 5' end the weak P\T promoter fused upstream of sequence encoding the lacZ reporter enzyme (the lacZ sequence is fused in-frame to the second exon of the P\T coding sequence). In addition, the endogenous (weak) Dmau\mariner\T promoter is also present approximately 700bp upstream of the enhancer trap sequence and could potentially effect lacZ expression.

(Lidholm et al., 1993)
Construct components
Component
Use(s)
Element type
Encoded product / tool
lacZ
Marker(s)
w+mW.hs
Component allele Dmau\mariner\TpA2L
Transgenic product class
Component
Use(s)
Encoded product / tool
Dmau\mariner\T
Description

Construct: Fragment of Mos1 up to nucleotide 787, including the Dmau\mariner 5' terminal repeat.

(Lidholm et al., 1993)

Dmau\mariner\T coding region interrupted at position 789 by introduction of w+mW.hs.

(Lohe et al., 1995)

Insertion of foreign DNA, including w+mW.hs, in the SacI site at position 789 of the Mos1 transposable element.

(Lohe and Hartl, 1996)
Component allele Ecol\lacZP\T.A92a
Transgenic product class
Component
Use(s)
Encoded product / tool
Ecol\lacZ
Description

Construct: In frame translational fusion of P\T to Ecol\lacZ, in the second exon of the P element. The fusion includes the P element promoter. The P-element 5' end is from pUChsneo.

(Wilson et al., 1989)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
epidermis
(Perrimon et al., 1991)
organism | 10-100% egg length
(Perrimon et al., 1991)
• nucleus
(Perrimon et al., 1991)
extended germ band stage
dorsal ectoderm | segmentally repeated
(Bellen et al., 1989)
embryonic stage 9 -- 12
procephalic neurogenic region
(Bellen et al., 1989)
Additional Information
Statement
Reference

Basal pattern of stripes, restricted to epidermal clusters of cells. Expression localized to nucleus.

(Perrimon et al., 1991)

Expression pattern dependent upon position of insertion in genome. Basal pattern observed in approximately 30% of transformants: in extended germ band embryos, weak expression in dorsal epidermis, stronger in alternate segments.

(Bellen et al., 1989)
 
Marker for
Reflects expression of
P\T
(Bellen et al., 1989)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pM{MlwB}
(Lidholm et al., 1993)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{MlwB}
(FlyBase, 1996-)
SacW
(Lozovsky et al., 2002)
Secondary FlyBase IDs
    References (6)