Insertion of foreign DNA, including w^+mW.hs, in the SacI site at position 789 of the Mos1 transposable element.

Dmau\mariner\T coding region interrupted at position 789 by introduction of w^+mW.hs.

Construct: Fragment of Mos1 up to nucleotide 787, including the Dmau\mariner 5' terminal repeat.

Assayed in D.melanogaster.

Assayed in D.melanogaster. Insertion of foreign DNA severely inhibits somatic excision of the transposon. Construct also exhibits great, though not complete, stability in the germline, even in the presence of a germline specific transposase source. Rare germline events of transposition and excision are identified. Examination of the excision products yields evidence of template-dependent gap repair, analogous to that previously reported for the P-element.