FB2024_03 , released June 25, 2024
Recombinant Construct: P{EcRE.lacZ}
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General Information
Symbol
P{EcRE.lacZ}
FlyBase ID
FBtp0001553
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZAdh.EcRE
Also Known As
EcRE-lacZ, 7xEcRE-lacZ, EcRE.lacZ
Size
Expression Data
lacZ reporter
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lacZAdh.EcRE
Transgenic product class
Component
Use(s)
Encoded product / tool
lacZ
Description

7 copies of a 30bp EcR element unit from the Hsp27 promoter (23bp element and 7 flanking bp) fused upstream of an Adh TATA box located within a BglII-ScaI fragment of the Adh distal promoter (-34 to +53) fused upstream of lacZ coding region.

(Koelle et al., 1991)

lacZ expression is driven by seven copies of the EcR response element.

(White et al., 1997)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 12
visceral mesoderm
(Chavoshi et al., 2010)
embryonic head
(Chavoshi et al., 2010)
embryonic stage 13 -- 14
amnioserosa
(Chavoshi et al., 2010)
third instar larval stage
eye disc morphogenetic furrow
(Brennan et al., 1998)
adult stage
adult brain
(Schwedes et al., 2011)
larval ventral nerve cord
(Schwedes et al., 2011)
adult fat body
(Schwedes et al., 2011)
adult oenocyte
(Schwedes et al., 2011)
adult proventriculus
(Schwedes et al., 2011)
adult midgut
(Schwedes et al., 2011)
adult hindgut
(Schwedes et al., 2011)
adult posterior hindgut
(Schwedes et al., 2011)
adult Malpighian tubule
(Schwedes et al., 2011)
adult stage | female
follicle cell
(Schwedes et al., 2011)
adult stage | male
male accessory gland
(Schwedes et al., 2011)
testis
(Schwedes et al., 2011)
seminal vesicle
(Schwedes et al., 2011)
ejaculatory bulb
(Schwedes et al., 2011)
ejaculatory duct
(Schwedes et al., 2011)
oogenesis stage S9
centripetal follicle cell
(Jang et al., 2009)
stretch follicle cell
(Jang et al., 2009)
Additional Information
Statement
Reference

The reporter was used to monitor EcR/usp activity in 0-, 5- and 10-day adults. Activity was observed in the Malpighian tubules and accessory glands at all times. Activity was rarely observed after day 0 in the CNS, abdominal cuticle fat, and seminal vesicles. Activity in adult oenocytes increased after day 0. Activity was observed within the first few hours after eclosion in testis but was absent by day 5. Activity in the ejaculatory bulb and duct varied over the time period. Activity was also observed in follicle cells and gut.

(Schwedes et al., 2011)

In third instar larval eye disc, expression restricted to a zone in and slightly anterior to the morphogenetic furrow; first observed when the morphogenetic furrow has produced 10-12 columns of ommatidia; travels with the furrow until the end of third instar, when it becomes ubiquitous.

(Brennan et al., 1998)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{EcRE.lacZ}
(Munroe et al., 1998.5.12)
descendant(s)
Comments

report comment: P{EcRE.lacZ} carries a reporter gene consisting of 7 copies of a 30bp sequence from Hsp27, which includes the 23-bp EcRE region; these are followed by the Adh distal promoter; followed by the lacZ gene.

(Munroe et al., 1998.5.12)
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
Bloomington
4517
w*; P{EcRE.lacZ}SS3
4516
y* w*; P{EcRE.lacZ}SS4
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
References (23)