FB2024_03 , released June 25, 2024
Allele: Ecol\lacZAdh.EcRE
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General Information
Symbol
Ecol\lacZAdh.EcRE
Species
E. coli
Name
FlyBase ID
FBal0057023
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{EcRE.lacZ}
Also Known As
EcRE-lacZ, 7xEcRE-lacZ, EcRE.lacZ
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Brennan et al., 1998, Munroe et al., 1998.5.12, White et al., 1997, Koelle et al., 1991)
Progenitor genotype
Carried in construct
P{EcRE.lacZ}
Cytology
Description

lacZ expression is driven by seven copies of the EcR response element.

(White et al., 1997)

7 copies of a 30bp EcR element unit from the Hsp27 promoter (23bp element and 7 flanking bp) fused upstream of an Adh TATA box located within a BglII-ScaI fragment of the Adh distal promoter (-34 to +53) fused upstream of lacZ coding region.

(Koelle et al., 1991)
Allele components
Component
Use(s)
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 12
      visceral mesoderm
      (Chavoshi et al., 2010)
      embryonic head
      (Chavoshi et al., 2010)
      embryonic stage 13 -- 14
      amnioserosa
      (Chavoshi et al., 2010)
      third instar larval stage
      eye disc morphogenetic furrow
      (Brennan et al., 1998)
      adult stage
      adult brain
      (Schwedes et al., 2011)
      larval ventral nerve cord
      (Schwedes et al., 2011)
      adult fat body
      (Schwedes et al., 2011)
      adult oenocyte
      (Schwedes et al., 2011)
      adult proventriculus
      (Schwedes et al., 2011)
      adult midgut
      (Schwedes et al., 2011)
      adult hindgut
      (Schwedes et al., 2011)
      adult posterior hindgut
      (Schwedes et al., 2011)
      adult Malpighian tubule
      (Schwedes et al., 2011)
      adult stage | female
      follicle cell
      (Schwedes et al., 2011)
      adult stage | male
      male accessory gland
      (Schwedes et al., 2011)
      testis
      (Schwedes et al., 2011)
      seminal vesicle
      (Schwedes et al., 2011)
      ejaculatory bulb
      (Schwedes et al., 2011)
      ejaculatory duct
      (Schwedes et al., 2011)
      oogenesis stage S9
      centripetal follicle cell
      (Jang et al., 2009)
      stretch follicle cell
      (Jang et al., 2009)
      Additional Information
      Statement
      Reference

      The reporter was used to monitor EcR/usp activity in 0-, 5- and 10-day adults. Activity was observed in the Malpighian tubules and accessory glands at all times. Activity was rarely observed after day 0 in the CNS, abdominal cuticle fat, and seminal vesicles. Activity in adult oenocytes increased after day 0. Activity was observed within the first few hours after eclosion in testis but was absent by day 5. Activity in the ejaculatory bulb and duct varied over the time period. Activity was also observed in follicle cells and gut.

      (Schwedes et al., 2011)

      In third instar larval eye disc, expression restricted to a zone in and slightly anterior to the morphogenetic furrow; first observed when the morphogenetic furrow has produced 10-12 columns of ommatidia; travels with the furrow until the end of third instar, when it becomes ubiquitous.

      (Brennan et al., 1998)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in reporter plasmid "pEcRE/Adh/βgal" and expressed in S2 cell line to assay for ecdysone binding activity mediated by EcR.

      (Koelle et al., 1991)

      Carried in reporter plasmid " 7xEcRE:LacZ ", expressed in S2 tissue culture cells and Hr46 repression of transactivation by EcR is studied.

      (White et al., 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ecol\lacZAdh.EcRE
      lacZAdh.EcRE
      lacZEcR.7
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0060289
      References (24)