FlyBase Recombinant Construct Report: P{UAS-RA.cs2}

General Information

Symbol

P{UAS-RA.cs2}

FlyBase ID

FBtp0001435

Feature type

transgenic_transposable_element

Component Allele(s)

Rcom\RT^A-cs2.UAS

Also Known As

UAS-ricin^CS, UAS-Ricin

Size

7.199

Expression Data

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Rcom\RT^A-cs2.UAS

Transgenic product class

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

RT-A(cs2)

temperature-regulated cell ablation tool

Description

UASt regulatory sequences drive expression of a cold-sensitive form of RT-A(cs2).

(Allen et al., 2002)

Sequence Data

Sequence (FB)

FlyBase-compiled

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

7.199

Left end

P5\'

(Allen et al., 1993)

Right end

P3\'

(Allen et al., 1993)

Segments

Num

Orientation

Symbol

CaSpeR_P5'(P5',Sp)

001186

5xUAS

Hsp70Bb::RA^cs2::SV40

001187

Car1_P3'(H3,P3')

Reference

(Allen et al., 1993)

Phenotypic Data

Component Allele

Phenotypic Data

Rcom\RT^A-cs2.UAS

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{UAS-RA.cs2}

(FlyBase, 1992-)

descendant(s)

Comments

construct_comment: RA^cs2 is a cold-sensitive mutation of the ricin toxin A chain (RA or RTA; from castor bean, Ricinus communis).Selectivity controlled both by temperature sensitivity and by use of different GAL4 regulatory constructs.

(Allen et al., 1993)

report comment: Included as non-attributed correction.

(Allen et al., 1993)

construct_comment: Selectable marker not indicated.

(Connolly et al., 1993)

Stocks (2)

Stocks containing insertion(s) of this recombinant construct

Bloomington

38623

w¹¹¹⁸; P{UAS-RA.cs2}39

38624

w¹¹¹⁸; P{UAS-RA.cs2}CC

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

P{UAS-RA.cs2}

(Chen and Chiang, 2012.5.22, FlyBase, 1996-)

P{UAS-RAcs2}

(FlyBase, 1996-)

UAS-RA.CS2

(González Morales et al., 2023)

UAS-RAcs2

(Allen et al., 2002)

UAS-RICIN

(Zhao et al., 2019)

UAS-RICIN^cs

(Pai et al., 2013)

UAS-Ricin

(Baena-López et al., 2008)

UAS-Ricin^CS

(Wang et al., 2023)

UAS-Ricin^cs

(Mesquita et al., 2010)

UAS-ricin^CS

(Chen et al., 2023, Wu et al., 2017, Chen et al., 2012)

UAS-ricin^ts

(Baena-López et al., 2008)

Secondary FlyBase IDs

FBtp0000731

References (18)

Report Sections

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