FB2024_03 , released June 25, 2024
Recombinant Construct: P{dppshv-lacZ.RD2}
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General Information
Symbol
P{dppshv-lacZ.RD2}
FlyBase ID
FBtp0001229
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZdpp.RD2
Also Known As
P{w+mW.hs,8.9dpp::LacZ}, RD2
Size
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
ry+t7.2
Component allele Ecol\lacZdpp.RD2
Transgenic product class
Component
Use(s)
Regulatory region(s)
dpp_RD2
Encoded product / tool
lacZ
Description

Fragment of dpp from coordinates 67 to 75.9 on the dpp map, i.e. the shv region, placed upstream of an Hsp70 promoter, and driving expression of lacZ.

(Hursh et al., 1993)

The 8.9kb EcoRI dpp visceral mesoderm (VMS) enhancer drives expression of lacZ.

(Miller et al., 2001)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
embryonic/larval midgut
(Heuer et al., 1995)
pupal stage
longitudinal vein
(Chauhan et al., 2013)
posterior crossvein
(Chauhan et al., 2013)
wing margin
(Chauhan et al., 2013)
pupal stage P6
wing vein

Comment: in longitudinal veins

(Christoforou et al., 2008)
pupal stage P6 -- P7
crossvein
(Christoforou et al., 2008)
Additional Information
Statement
Reference

Reporter expression is first observed in the longitudinal veins at 19 hr APF. In crossveins, it is first observed at about 29 hr APF, is prominent by 36hr APF, and persists until adult cuticle is secreted.

(Christoforou et al., 2008)
 
Marker for
Reflects expression of
dpp
(Chauhan et al., 2013, Christoforou et al., 2008)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{dppshv-lacZ.RD2}
(Hursh et al., 1993)
descendant(s)
Comments
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
P{dppshv-lacZ.RD2}
(FlyBase, 1996-)
P{w+mW.hs,8.9dpp::LacZ}
(Miller et al., 2001, Miller et al., 2001)
RD2
(Heuer et al., 1995, Hursh et al., 1993)
RD2-lacZ
(Christoforou et al., 2008)
dppshv-lacZ.RD2
(Chauhan et al., 2013)
Secondary FlyBase IDs
    References (11)