FB2024_03 , released June 25, 2024
Allele: Ecol\lacZdpp.RD2
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General Information
Symbol
Ecol\lacZdpp.RD2
Species
E. coli
Name
FlyBase ID
FBal0041163
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{dppshv-lacZ.RD2}
Also Known As
P{w+mW.hs,8.9dpp::LacZ}, RD2
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Hursh et al., 1993)
Progenitor genotype
Carried in construct
P{dppshv-lacZ.RD2}
Cytology
Description

The 8.9kb EcoRI dpp visceral mesoderm (VMS) enhancer drives expression of lacZ.

(Miller et al., 2001)

Fragment of dpp from coordinates 67 to 75.9 on the dpp map, i.e. the shv region, placed upstream of an Hsp70 promoter, and driving expression of lacZ.

(Hursh et al., 1993)
Allele components
Component
Use(s)
Regulatory region(s)
dpp_RD2
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      embryonic/larval midgut
      (Heuer et al., 1995)
      pupal stage
      longitudinal vein
      (Chauhan et al., 2013)
      posterior crossvein
      (Chauhan et al., 2013)
      wing margin
      (Chauhan et al., 2013)
      pupal stage P6
      wing vein

      Comment: in longitudinal veins

      (Christoforou et al., 2008)
      pupal stage P6 -- P7
      crossvein
      (Christoforou et al., 2008)
      Additional Information
      Statement
      Reference

      Reporter expression is first observed in the longitudinal veins at 19 hr APF. In crossveins, it is first observed at about 29 hr APF, is prominent by 36hr APF, and persists until adult cuticle is secreted.

      (Christoforou et al., 2008)
       
      Marker for
      Reflects expression of
      dpp
      (Chauhan et al., 2013, Christoforou et al., 2008)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Fragment of dpp cis regulatory region that drives Ecol\lacZ expression includes the 812bp regulatory region that directs expression in parasegment 7 of the visceral mesoderm.

      (Sun et al., 1995)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ecol\lacZdpp.RD2
      lacZdpp.RD2
      Name Synonyms
      Secondary FlyBase IDs
        References (11)