FB2024_03 , released June 25, 2024
Recombinant Construct: P{5xglBS-lacZ.38-1}
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General Information
Symbol
P{5xglBS-lacZ.38-1}
FlyBase ID
FBtp0001226
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZ5xglBS.38-1
Also Known As
glass-lacZ
Size
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
ry+t7.2
Component allele Ecol\lacZ5xglBS.38-1
Transgenic product class
Component
Use(s)
Encoded product / tool
lacZ
Description

A pentamer of a 38bp sequence (that includes a gl protein-binding site plus additional flanking nucleotides) from the ninaE element II enhancer is fused upstream of an Hsp70 promoter truncated to position -50bp followed by lacZ.

(Moses and Rubin, 1991)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
Bolwig nerve
(Moses and Rubin, 1991)
photoreceptor neuron | presumptive
(Ellis et al., 1993)
embryonic/larval optic stalk
(Moses and Rubin, 1991)
third instar larval stage
eye-antennal disc
(Ellis et al., 1993)
eye-antennal disc | posterior
(Moses and Rubin, 1991)
photoreceptor neuron | presumptive
(Ellis et al., 1993, Moses and Rubin, 1991)
eye-antennal disc | restricted
(Moses and Rubin, 1991)
embryonic/larval brain | restricted
(Ellis et al., 1993)
eye disc morphogenetic furrow | posterior
(Moses and Rubin, 1991)
Additional Information
Statement
Reference

In third instar larva: expressed predominantly in photoreceptor cells in eye-antennal disc; also expressed in a small number of cells in the brain.

(Ellis et al., 1993)

The reporter construct is expressed only in photoreceptor cells in the developing eye disc.

(Ellis et al., 1993)

In the eye disc, reporter geneexpression is found only in the posterior part of the disc where it isrestricted to photoreceptor cells. Expression is also seen in the opticstalk and in Bolwig's nerve.

(Moses and Rubin, 1991)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

construct_comment: One of a series of lacZ reporter constructs used to assay function of ninaE promoter regions that contain glass protein binding sites.

(Ellis et al., 1993)

construct_comment: See report for GMR.

(Moses and Rubin, 1991)
Stocks (760)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (16)