FlyBase Allele Report: Ecol\lacZ[5xglBS.38-1]

General Information

Symbol

Ecol\lacZ^5xglBS.38-1

Species

E. coli

Name

FlyBase ID

FBal0041801

Feature type

allele

Associated gene

Ecol\lacZ

Associated Insertion(s)

Carried in Construct

P{5xglBS-lacZ.38-1}

Also Known As

glass-lacZ

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Ellis et al., 1993, Moses and Rubin, 1991)

Progenitor genotype

Carried in construct

P{5xglBS-lacZ.38-1}

Cytology

Description

A pentamer of a 38bp sequence (that includes a gl protein-binding site plus additional flanking nucleotides) from the ninaE element II enhancer is fused upstream of an Hsp70 promoter truncated to position -50bp followed by lacZ.

(Moses and Rubin, 1991)

Allele components

Component

Use(s)

Encoded product / tool

lacZ

reporter enzyme

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

Bolwig nerve

(Moses and Rubin, 1991)

photoreceptor neuron | presumptive

(Ellis et al., 1993)

embryonic/larval optic stalk

(Moses and Rubin, 1991)

third instar larval stage

eye-antennal disc

(Ellis et al., 1993)

eye-antennal disc | posterior

(Moses and Rubin, 1991)

photoreceptor neuron | presumptive

(Ellis et al., 1993, Moses and Rubin, 1991)

eye-antennal disc | restricted

(Moses and Rubin, 1991)

embryonic/larval brain | restricted

(Ellis et al., 1993)

eye disc morphogenetic furrow | posterior

(Moses and Rubin, 1991)

Additional Information

Statement

Reference

In third instar larva: expressed predominantly in photoreceptor cells in eye-antennal disc; also expressed in a small number of cells in the brain.

(Ellis et al., 1993)

The reporter construct is expressed only in photoreceptor cells in the developing eye disc.

(Ellis et al., 1993)

In the eye disc, reporter geneexpression is found only in the posterior part of the disc where it isrestricted to photoreceptor cells. Expression is also seen in the opticstalk and in Bolwig's nerve.

(Moses and Rubin, 1991)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease