FB2024_03 , released June 25, 2024
Recombinant Construct: P{5xglBS-hs-lacZ.A}
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General Information
Symbol
P{5xglBS-hs-lacZ.A}
FlyBase ID
FBtp0001223
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZ5xglBS.hs.A
Size
Expression Data
lacZ reporter
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
ry+t7.2
Component allele Ecol\lacZ5xglBS.hs.A
Transgenic product class
Component
Use(s)
Regulatory region(s)
ninaE_constructA
Encoded product / tool
lacZ
Description

lacZ reporter expression is regulated by ninaE enhancer element II (including a pentamer of gl binding sites) and an Hsp70 promoter truncated to position -250bp.

(Moses and Rubin, 1991)

A pentamer of a 38bp sequence (that includes a gl protein-binding site plus additional flanking nucleotides) from the ninaE element II enhancer is fused upstream of an Hsp70 promoter truncated to position -250bp (this is sufficient for heat inducibility) followed by lacZ.

(Moses and Rubin, 1991)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
embryonic/larval brain
(Moses and Rubin, 1991)
photoreceptor neuron | presumptive
(Moses and Rubin, 1991)
eye disc morphogenetic furrow
(Moses and Rubin, 1991)
eye-antennal disc | posterior
(Moses and Rubin, 1991)
adult stage
ocellus
(Moses and Rubin, 1991)
retina
(Moses and Rubin, 1991)
Additional Information
Statement
Reference

Reporter gene expression is observed in a three zone pattern in the eye imaginal disc. No expression is seen anterior to the furrow, weak expression begins in the furrow and extends posteriorly, and strong expression occurs from about the 8th column of developing ommatidia to the back of the disc. Expression occurs only in the photoreceptor cells.

(Moses and Rubin, 1991)

No expression in adult brain.

(Moses and Rubin, 1991)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

construct_comment: See report for GMR.

(Moses and Rubin, 1991)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Construct A
(Moses and Rubin, 1991)
P{5xglBS-hs-lacZ.A}
(FlyBase, 1996-)
P{GMR-lacZ.A}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)