FB2024_03 , released June 25, 2024
Allele: Ecol\lacZ5xglBS.hs.A
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General Information
Symbol
Ecol\lacZ5xglBS.hs.A
Species
E. coli
Name
FlyBase ID
FBal0041800
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{5xglBS-hs-lacZ.A}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Moses and Rubin, 1991)
Progenitor genotype
Carried in construct
P{5xglBS-hs-lacZ.A}
Cytology
Description

lacZ reporter expression is regulated by ninaE enhancer element II (including a pentamer of gl binding sites) and an Hsp70 promoter truncated to position -250bp.

(Moses and Rubin, 1991)

A pentamer of a 38bp sequence (that includes a gl protein-binding site plus additional flanking nucleotides) from the ninaE element II enhancer is fused upstream of an Hsp70 promoter truncated to position -250bp (this is sufficient for heat inducibility) followed by lacZ.

(Moses and Rubin, 1991)
Allele components
Component
Use(s)
Regulatory region(s)
ninaE_constructA
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      embryonic/larval brain
      (Moses and Rubin, 1991)
      photoreceptor neuron | presumptive
      (Moses and Rubin, 1991)
      eye disc morphogenetic furrow
      (Moses and Rubin, 1991)
      eye-antennal disc | posterior
      (Moses and Rubin, 1991)
      adult stage
      ocellus
      (Moses and Rubin, 1991)
      retina
      (Moses and Rubin, 1991)
      Additional Information
      Statement
      Reference

      Reporter gene expression is observed in a three zone pattern in the eye imaginal disc. No expression is seen anterior to the furrow, weak expression begins in the furrow and extends posteriorly, and strong expression occurs from about the 8th column of developing ommatidia to the back of the disc. Expression occurs only in the photoreceptor cells.

      (Moses and Rubin, 1991)

      No expression in adult brain.

      (Moses and Rubin, 1991)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ecol\lacZ5xglBS.hs.A
      Ecol\lacZGMR.A.hs
      lacZGMR.A.hs
      Name Synonyms
      Secondary FlyBase IDs
        References (2)