FlyBase Recombinant Construct Report: P{hsp70-lacZ}

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Recombinant Construct: P{hsp70-lacZ}

General Information

Symbol

P{hsp70-lacZ}

FlyBase ID

FBtp0000170

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^Hsp70Bb.-194

Also Known As

(hsp70-lacZ) Bg⁹, Hsp70-lacZ

Size

18.154

Expression Data

lacZ reporter

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

Component allele Ecol\lacZ^Hsp70Bb.-194

Transgenic product class

Component

Use(s)

Encoded product / tool

reporter enzyme

Description

Hsp70Bb promoter sequences from 194bp upstream of the transcription start site and some Hsp70Bb coding sequence is fused to lacZ.

(Lis et al., 1983)

Hsp70 promoter sequences from position -194 to +1250 are fused in frame to lacZ.

(Vincent et al., 1990)

Component allele l(3)87Df^+t8.1

Transgenic product class

(Dutton and Chovnick, 1991)

(Dutton and Chovnick, 1991)

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Description

8.2kb genomic SalI fragment that includes the l(3)87Df gene.

(Dutton and Chovnick, 1991)

Sequence Data

Sequence (FB)

FlyBase-compiled

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

18.154

Left end

P5\'

(Lis et al., 1983)

Right end

P3\'

(Lis et al., 1983)

Segments

Num

Orientation

Symbol

1

+

6.1_P5'(P5',X1)

2

+

3

-

4

+

5

-

Hsp70::lacZ(ep19.1)

6

+

7

+

6.1_P3'(X1,P3')

Reference

(Lis et al., 1983)

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^Hsp70Bb.-194

Phenotypic Class Phenotype Manifest In Detailed Description

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Papaconstantinou et al., 2005)

organism | ubiquitous

(Lis et al., 1983)

organism | ubiquitous

(Lis et al., 1983)

adult stage & oogenesis

(Lis et al., 1983)

Additional Information

Statement

Reference

Subsequent to heat-shock, high levels of expression observed in most tissues. Higher levels observed in early egg chambers relative to late egg chambers. Quantitation hindered by variable permeability of different structures and tissues, affecting uptake of the X-gal substrate. Without heat-shock, expression cannot be distinguished from background levels.

(Lis et al., 1983)

Marker for

Reflects expression of

(Papaconstantinou et al., 2005)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

(Lis et al., 1983)

descendant(s)

Comments

construct_comment: Produces new heat-inducible puff at cytological site of insertion.

(Lis et al., 1983)

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

(hsp70-lacZ) Bg⁹

(Shakya et al., 2012, Nazir et al., 2003)

Bg9

(Lakhotia et al., 2002)

Bg⁹

(Sharma et al., 2011)

Hsp70-lacZ

(Papaconstantinou et al., 2005)

P{hsp70-lacZ}

(FlyBase, 1996-)

ep19.1

(FlyBase, 1996-)

hsp70-lacZ

(Sharma et al., 2011)

hsp70:lacZ

(Bhole et al., 2004)

Secondary FlyBase IDs

References (18)

Report Sections