FB2024_03 , released June 25, 2024
Allele: Ecol\lacZHsp70Bb.-194
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General Information
Symbol
Ecol\lacZHsp70Bb.-194
Species
E. coli
Name
FlyBase ID
FBal0038869
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{HBΔ-194}
P{hsp70-lacZ}
P{SXhLac-7}
Also Known As
(hsp70-lacZ) Bg9, Hsp70-lacZ
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Helfand and Naprta, 1996, Lakhotia, 1994, Weeks et al., 1993, Vincent et al., 1990, Lis et al., 1983)
Progenitor genotype
Carried in construct
P{HBΔ-194}
P{hsp70-lacZ}
P{SXhLac-7}
Cytology
Description

Hsp70 promoter sequences from position -194 to +1250 are fused in frame to lacZ.

(Vincent et al., 1990)

Hsp70Bb promoter sequences from 194bp upstream of the transcription start site and some Hsp70Bb coding sequence is fused to lacZ.

(Lis et al., 1983)
Allele components
Component
Use(s)
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism | ubiquitous
      (Papaconstantinou et al., 2005)
      larval stage
      organism | ubiquitous
      (Lis et al., 1983)
      adult stage
      organism | ubiquitous
      (Lis et al., 1983)
      adult stage & oogenesis
      egg chamber
      (Lis et al., 1983)
      Additional Information
      Statement
      Reference

      Subsequent to heat-shock, high levels of expression observed in most tissues. Higher levels observed in early egg chambers relative to late egg chambers. Quantitation hindered by variable permeability of different structures and tissues, affecting uptake of the X-gal substrate. Without heat-shock, expression cannot be distinguished from background levels.

      (Lis et al., 1983)
       
      Marker for
      Reflects expression of
      Hsp70Bb
      (Papaconstantinou et al., 2005)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Lis

      (Weeks et al., 1993)
      Comments
      Comments

      Carried in plasmid "ep19.1".

      (Lis et al., 1983)

      Large puffs are formed in response to heat shock.

      (Simon et al., 1985)

      Posttranscriptional upregulation during aging. Expression during aging is specific to the flight muscles and leg muscles. The time-course of expression is proportional to life-span.

      (Wheeler et al., 1995)

      The rate and amount of induction of Ecol\lacZ protein after heat shock is preserved during aging, but the rate of decay of Ecol\lacZ protein may be slightly delayed in older animals.

      (Helfand and Naprta, 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ecol\lacZHsp70Bb.-194
      lacZHsp70Bb.-194
      lacZhs.ep19.1
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0044070
      • FBal0044080
      References (30)