A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.
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Comment: 24-55h APF
In brains taken from 48hr APF pupae, ScerGAL4caps-NP3294 is variably expressed across antennal lobe glomeruli, with some expressing high levels and others low.
ScerGAL4caps-NP3294 drives expression in the medulla. At late pupal stage, the terminals of photoreceptor R8 are located in a layer of caps expression, whereas the terminals of R7 are in a caps-negative layer.
Location 3L:13202569-13202670 determined by FlyBase alignment of dbGSS accession AG217321 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.