A set of UAS-ORF transgenic constructs that are designed for use in a bimolecular fluorescence complementation (BiFC) assay. Each transgenic construct carries an ORF of interest tagged with a C-terminal C-Cerulean split fluorescent protein fragment and preceded by UASt regulatory sequences. All constructs also carry the w+mC mini-white marker. Controlled expression of the ORFs can be effected by introduction of GAL4 drivers.