FB2024_03 , released June 25, 2024
Reagent: FlyORF-CC
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General Information
Name
FlyORF-CC
Species
D. melanogaster
Reagent type
construct collection
FlyBase ID
FBlc0003487
Project
Created by
Vector
Title
A set of transgenic constructs for expression of C-terminal C-Cerulean-tagged protein coding sequences under UAS control.
Accessions
    Overview
    Description

    A set of UAS-ORF transgenic constructs that are designed for use in a bimolecular fluorescence complementation (BiFC) assay. Each transgenic construct carries an ORF of interest tagged with a C-terminal C-Cerulean split fluorescent protein fragment and preceded by UASt regulatory sequences. All constructs also carry the w+mC mini-white marker. Controlled expression of the ORFs can be effected by introduction of GAL4 drivers.

    (Bischof et al., 2018)
    Available from
    [FlyORF](http://flyorf.ch/index.php)
    (Bischof et al., 2018)
    Biosample Source
    Overview
    Strain
    Stage
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Reagent Details
    Methods
    Transgenic Construct used
    Molecular Construct used
    Protocol

    ORFs were cloned into the pGW-CC.attB vector using Gateway cloning. Pools of ORF clones were injected into embryos of a strain carrying M{3xP3-RFP.attP}ZH-21F (an attP target site on the second chromosome).

    (Bischof et al., 2018)
    Mode of Assay
    Data analysis
    Comments
    Associated Data
    Size
    Associated features
    328 Insertion(s)
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    Additional Information
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    FlyORF-CC
    Name Synonyms
    A set of transgenic constructs for expression of C-terminal C-Cerulean-tagged protein coding sequences under UAS control.
    (Bischof et al., 2018)
    Secondary FlyBase IDs
      References (1)