FB2024_03 , released June 25, 2024
Recombinant Construct: pGW-CC.attB
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General Information
Symbol
pGW-CC.attB
FlyBase ID
FBmc0003185
Feature type
engineered_plasmid
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

The pGW-CC.attB plasmid is designed for generating donor plasmids that can be used to insert sequence containing an open reading frame (ORF) of interest, expressed under the control of UASt regulatory sequences and tagged at the C-terminal end with C-Cerulean, into an attP-containing docking site in the genome, via phiC31:int-mediated integration. A loxP site is present upstream of the UASt sequences and an FRT5 site is present in between the UASt and ORF sequences. An ORF of interest can be inserted into the plasmid via a Gateway cassette.

(Bischof et al., 2018)
Construct components
Component
Use(s)
Regulatory region(s)
UASt
Tagged with
C-Cerulean
Also carries
FRT5
loxP
Marker(s)
w+mC
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
pGW-CC.attB
(Bischof et al., 2018, FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)