A set of transgenic insertion stocks containing a gene trap cassette, derived from Mi{MIC} insertions in coding introns using Recombinase-Mediated Cassette Exchange (RMCE); see http://flypush.imgen.bcm.tmc.edu/pscreen/technique.html. The gene trap cassette contains a Trojan GAL4 exon composed of a splice acceptor, T2A peptide, GAL4 coding sequence and an Hsp70 transcription termination signal. When inserted in a coding intron in the correct orientation, the cassette should result in truncation of the trapped gene product and expression of GAL4 under the control of the regulatory sequences of the trapped gene. Each insertion line contains one of three versions of the cassette (Mi{Trojan-GAL4.0}, Mi{Trojan-GAL4.1} or Mi{Trojan-GAL4.2}), depending on the frame required to generate a gene trap.
The translational mbl reporter is expressed in third instar larval photoreceptors. In the brain, expression is observed mainly in post-mitotic neurons with some expression in glial cells. It is also detected in muscles. An intersectional strategy was used to label single cells. mbl reporter is observed in L1, L4, R7 and R8 neurons. mbl is expressed in a cell-specific manner that correlates with the expression of Dscam2 exon 10B but not 10A. It is not detected in mushroom body neurons that express Dscam2 exon 10A in adults.
MI00139-TG4.0
