FlyBase Insertion Report: Dmel\Mi{Trojan-GAL4DBD.0}ChAT[MI04508-TG4DBD.0]

General Information

Symbol

Dmel\Mi{Trojan-GAL4DBD.0}ChAT^{MI04508-TG4DBD.0}

Species

D. melanogaster

Name

FlyBase ID

FBti0168158

Feature type

transposable_element_insertion_site

Also Known As

ChAT-GAL4DBD, ChAT-DBD, ChaT^MI04508-Gal4DBD

Description

Inserted Element

Mi{Trojan-GAL4DBD.0}

LINE ID

Affected gene(s)

CG7715, ChAT

Viability / fertility

Associated allele(s)

CG7715^{MI04508-TG4DBD.0-X}, ChAT^{MI04508-TG4DBD.0}, Scer\GAL4^{ChAT-MI04508-TG4DBD.0}

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 91C1-91C5 )

Sequence Location

Target / Docking site

Mi{MIC}ChAT^MI04508

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

91C1-91C5 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

Mi{Trojan-GAL4DBD.0}

(Diao et al., 2015)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

CG7715

(FlyBase, 1996-)

ChAT

(Diao et al., 2016, Diao et al., 2015)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

CG7715^{MI04508-TG4DBD.0-X}

Phenotypic Class Phenotype Manifest in Detailed Description

ChAT^{MI04508-TG4DBD.0}

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^{ChAT-MI04508-TG4DBD.0}

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult doublesex pCd (male) neuron

Comment: when combined with P{R41A01-p65.AD} (combination referred to as 'Chat ∩ R41A01')

(Jung et al., 2020)

neuron | subset of adult central nervous system

Comment: when combined with P{R41A01-p65.AD} (combination referred to as 'Chat ∩ R41A01')

(Jung et al., 2020)

mechanosensory neuron of leg chordotonal organ

Comment: when combined with TI{2A-p65(AD)::Zip+}unc-4^AD

(Lacin et al., 2020)

sensory neuron of campaniform sensillum

Comment: when combined with TI{2A-p65(AD)::Zip+}unc-4^AD

(Lacin et al., 2020)

Additional Information

Statement

Reference

Marker for

Reflects expression of

ChAT

(Lacin et al., 2020)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Replacement descendant of (target)

Mi{MIC}ChAT^MI04508

(Diao et al., 2015)

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

60318

w^*; l(2)*^*/CyO; Mi{Trojan-GAL4DBD.0}ChAT^{MI04508-TG4DBD.0} CG7715^{MI04508-TG4DBD.0-X}/TM3, Sb¹

External Crossreferences and Linkouts ( 0 )

Comments

phiC31-mediated recombination has been used to replace the attP cassette of the Mi{MIC} element with the intron phase 0 "Trojan GAL4DBD" cassette from the pBS-KS-attB2-SA(0)-T2A-GAL4DBD-Hsp70 plasmid.

(Diao et al., 2015)

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

ChAT-DBD

(Jung et al., 2020, Lacin et al., 2019)

ChAT-GAL4DBD

(Riabinina et al., 2019)

ChAT-Gal4(DBD)

(Katheder et al., 2023)

ChATDBD

(Lacin et al., 2019)

ChaT-GAL4DBD

(Diao et al., 2024)

ChaT^MI04508-Gal4DBD

(Diao et al., 2024, Diao et al., 2016)

Mi{Trojan-GAL4DBD.0}ChAT^{MI04508-TG4DBD.0}

Mi{Trojan-Gal4.DBD.0}ChAT^{MI04508-TG4DBD.0}

(Katheder et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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