FB2024_03 , released June 25, 2024
Insertion: Dmel\M{UAS-Tip60.ORF.3xHA.GW}ZH-86Fb
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General Information
Symbol
Dmel\M{UAS-Tip60.ORF.3xHA.GW}ZH-86Fb
Species
D. melanogaster
Name
FlyBase ID
FBti0161828
Feature type
transposable_element_insertion_site
Description
Inserted Element
M{UAS-Tip60.ORF.3xHA.GW}
LINE ID
Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
Sequence Location
Target / Docking site
M{3xP3-RFP.attP}ZH-86Fb
Member of Large Scale Dataset(s)
Dataset
FlyORF-3
Description

A collection of stocks of UAS-ORF transgenic recombinants that carry the ORF of interest marked by a C-terminal HA epitope tag and preceded by Scer\UAS sequences, Hsp70 promoter sequences and a Kozak consensus sequence for efficient translation. All constructs also carry the w+mC mini-white marker, and all have been introduced by recombination into a site at 86F on the third chromosome. Controlled expression of the ORFs can be effected by introduction of Scer\GAL4 drivers.

(Bischof et al., 2014)

See related datasets, FlyORF-2 and FlyORF-1.

(Bischof et al., 2013)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Affected Gene(s)
Insertion may affect gene
Variant Molecular Consequences
Phenotypic Data
Associated Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

The presence of incompatible Scer\FRT sites located upstream (FRT5 site) and downstream (FRT2 site) of the open reading frame in the inserted M{UAS-Tip60.ORF.3xHA.GW} element allows exchange of either the promoter or epitope tag sequences by FLP/FRT-mediated in vivo exchange.

(Bischof and FlyORF project members, 2014.6.20)
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
M{UAS-Tip60.ORF.3xHA.GW}ZH-86Fb
(Bischof and FlyORF project members, 2014.6.20)
Name Synonyms
Secondary FlyBase IDs
    References (1)