FlyBase Insertion Report: Dmel\P{GawB}gcm[rA87.P]

General Information

Symbol

Dmel\P{GawB}gcm^rA87.P

Species

D. melanogaster

Name

FlyBase ID

FBti0130081

Feature type

transposable_element_insertion_site

Also Known As

gcm-GAL4, gcmGal4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

gcm, Scer\GAL4

Viability / fertility

Associated allele(s)

gcm^rA87.P, Scer\GAL4^gcm-rA87.P

Stock availability

None publicly available

Genomic Location

Chromosomal Location

2L ( 30B12 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

30B12 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(Paladi and Tepass, 2004)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

gcm

(Cattenoz et al., 2016, Popkova et al., 2012, Jacques et al., 2009, Soustelle and Giangrande, 2007)

Scer\GAL4

(Popkova et al., 2012, Bunt et al., 2010, Ho et al., 2009, Jacques et al., 2009, Martinek et al., 2008, Soustelle and Giangrande, 2007, Paladi and Tepass, 2004)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

gcm^rA87.P

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^gcm-rA87.P

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 11

hemocyte

(Cho et al., 2002)

embryonic stage 8

head mesoderm

(Trébuchet et al., 2019)

embryonic stage 11 -- 17

hemocyte

(Sears et al., 2003)

embryonic stage 14

glial cell | subset

(Freeman et al., 2003)

first instar larval stage -- third instar larval stage

larval prothoracic A-subperineurial glial cell

(Soustelle and Giangrande, 2007)

larval mesothoracic A-subperineurial glial cell

(Soustelle and Giangrande, 2007)

larval metathoracic A-subperineurial glial cell

(Soustelle and Giangrande, 2007)

third instar larval stage

adult medulla neuropil associated glial cell

(Soustelle and Giangrande, 2007)

embryonic/larval brain | restricted

(Soustelle and Giangrande, 2007)

larval ventral midline glial cell

(Soustelle and Giangrande, 2007)

larval ventral nerve cord | restricted

Comment: four pairs of neurons within the dorsal region

(Soustelle and Giangrande, 2007)

pupal stage

presumptive spermatheca

(Cattenoz et al., 2016)

adult stage | female

spermatheca

(Cattenoz et al., 2016)

Additional Information

Statement

Reference

gcm reporter is expressed early in the development of the secretory cells of the spermathecum during pupal stages. In adults, reporter is detected in the spermathecum but not in the ovaries or oviduct.

(Cattenoz et al., 2016)

In addition to expression in glial precursor cells and lamina precursor cells in the third instar larval optic lobe and a central brain cluster, reporter expression is observed in medulla neuropil glial cells. Reporter expression is also observed in in the ventral nerve cord in leg neuropile glia and in four pairs of neurons within the dorsal region of the ventral nerve cord.

(Soustelle and Giangrande, 2007)

Embryonic expression is observed in all non-midline glial cells.

(Freeman et al., 2003)

Marker for

glial cell

(Freeman et al., 2003)

hemocyte

(Sears et al., 2003, Cho et al., 2002)

Reflects expression of

gcm

(Cattenoz et al., 2016, Soustelle and Giangrande, 2007, Freeman et al., 2003, Sears et al., 2003, Cho et al., 2002)

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Soustelle and Giangrande, 2007)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Recombinant descendant of (donor)

P{GawB}E132

(Paladi and Tepass, 2004)

Transposed descendant of

P{PZ}gcm^rA87

(Paladi and Tepass, 2004)

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

GCM-Gal4

(Sun et al., 2006)

Gcm-Gal4

(Sears et al., 2003)

P{GawB}gcm^rA87.P

gcm-GAL4

(Ho et al., 2009, Martinek et al., 2008, Freeman et al., 2003, Cho et al., 2002)

gcm-Gal4

(Popkova et al., 2012, Paladi and Tepass, 2004)

gcm-gal4

(Jacques et al., 2009, Soustelle and Giangrande, 2007)

gcmGal4

(Bazzi et al., 2023, Trébuchet et al., 2019, Cattenoz et al., 2016, Cattenoz et al., 2016)

glial cells missingGal4

(Bunt et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

Open Close