FlyBase Insertion Report: Dmel\P{GawB}gcm[rA87.C]

General Information

Symbol

Dmel\P{GawB}gcm^rA87.C

Species

D. melanogaster

Name

FlyBase ID

FBti0076768

Feature type

transposable_element_insertion_site

Also Known As

gcm-Gal4, gcmGal4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

gcm, Scer\GAL4

Viability / fertility

Associated allele(s)

gcm^rA87.C, Scer\GAL4^gcm-rA87.C

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

P{PZ}gcm^rA87

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(Chotard et al., 2005)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

gcm

(Chotard et al., 2005)

Scer\GAL4

(Hakeda-Suzuki et al., 2011, Chen and Clandinin, 2008, Weake et al., 2008, Pereanu et al., 2007, Soller et al., 2006, Chotard et al., 2005, Fan et al., 2005, Pereanu et al., 2005, Ting et al., 2005, Dumstrei et al., 2003)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

gcm^rA87.C

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^gcm-rA87.C

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval glial cell

(Reitman et al., 2015)

embryonic/larval hemocyte

(Reitman et al., 2015)

embryonic stage 11 -- first instar larval stage

glial cell

(Dumstrei et al., 2003)

larval stage -- pupal stage

neuropil glial cell

(Kato et al., 2020)

third instar larval stage

developing marginal glial cell

(Chotard et al., 2005)

epithelial glial cell | precursor

(Chotard et al., 2005)

lamina anlage

(Chotard et al., 2005, Ting et al., 2005)

adult medulla neuropil associated glial cell

(Chotard et al., 2005)

epithelial glial cell

(Chotard et al., 2005)

adult lamina marginal glial cell

(Chotard et al., 2005)

lamina monopolar neuron

(Chotard et al., 2005)

lamina monopolar neuron L1

(Chotard et al., 2005, Ting et al., 2005)

lamina monopolar neuron L2

(Chotard et al., 2005, Ting et al., 2005)

lamina monopolar neuron L3

(Chotard et al., 2005, Ting et al., 2005)

lamina monopolar neuron L4

(Chotard et al., 2005, Ting et al., 2005)

lamina monopolar neuron L5

(Chotard et al., 2005, Ting et al., 2005)

lamina anlage glial cell

(Chotard et al., 2005)

embryonic/larval eye disc marginal glial cell | precursor

(Chotard et al., 2005)

embryonic/larval eye disc marginal glial cell

(Chotard et al., 2005)

embryonic/larval epithelial glial cell | precursor

(Chotard et al., 2005)

embryonic/larval epithelial glial cell

(Chotard et al., 2005)

lamina furrow | posterior to

(Chotard et al., 2005)

lamina forming neuroblast

(Chotard et al., 2005)

medulla

(Chotard et al., 2005)

Additional Information

Statement

Reference

Drives expression in glial cells and in lamina monopolar neurons.

(FlyBase Curators, 2017)

ScerGAL4^gcm-rA87.C drives expression in lamina precursor cells, and can be used to label the axons of lamina monopolar neurons L1-L5.

(Ting et al., 2005)

In the larval visual system, ScerGAL4^gcm-rA87.C drives expression not only in specific glial subtypes, but also in the lineage that gives rise to lamina neurons. ScerGAL4^gcm-rA87.C drives expression in epithelial glia, marginal glia, and some medulla neuropil glia, but not in medulla or lobula glia.

(Chotard et al., 2005)

Reportor expression is observed in clusters of cells within GPC areas that have accumulated at the margins of the lamina that will become epithelial and marginal glial cells and also in some medulla neuropil glia. Expression is also observed in lamina precursor cells posterior to the lamina furrow, differentiated lamina neurons L1 through L5 and some medulla neurons.

(Chotard et al., 2005)

ScerGAL4^gcm-rA87.C drives expression in all glial cells at embryonic and early larval stages.

(Dumstrei et al., 2003)

Marker for

Reflects expression of

gcm

(Reitman et al., 2015, Chotard et al., 2005, Ting et al., 2005, Dumstrei et al., 2003)

Reporter construct used in assay

Avic\GFP^UAS.cUa

(Dumstrei et al., 2003)

Ecol\lacZ^UAS.cUa

(Ting et al., 2005)

Mmus\Cd8a^UAS.cLa.EGFP

(Chotard et al., 2005, Ting et al., 2005)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Replacement descendant of (target)

P{PZ}gcm^rA87

(Chotard et al., 2005)

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

35541

y¹ w^*; P{GawB}gcm^rA87.C/CyO

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

GCM-GAL4

(Miao et al., 2024)

GCM-Gal4

(Agi et al., 2024)

Gcm-Gal4

(Reitman et al., 2015, Ting et al., 2005)

Gcm-gal4

(He et al., 2019)

P{GawB}gcm^rA87.C

gcm-GAL4

(Bostock et al., 2020, Kato et al., 2020, Reitman et al., 2015, Spéder and Brand, 2014, Weake et al., 2008, Fan et al., 2005)

gcm-Gal4

(Lapraz et al., 2023, Schwabe et al., 2013, Hakeda-Suzuki et al., 2011, Pereanu et al., 2007, Chotard et al., 2005, Pereanu et al., 2005, Dumstrei et al., 2003)

gcmGal4

(Ayoub et al., 2023, Chen and Clandinin, 2008, Soller et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections