FlyBase Insertion Report: Dmel\P{GawB}NP1227

General Information

Symbol

Dmel\P{GawB}NP1227

Species

D. melanogaster

Name

FlyBase ID

FBti0034292

Feature type

transposable_element_insertion_site

Also Known As

LN1-Gal4, NP1227, NP1227-Gal4, Gal4-LN1, LN1Gal4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^NP1227

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG215184

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

(Das et al., 2008)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Sudhakaran et al., 2012, Acebes et al., 2011, Lieber et al., 2011, Thum et al., 2011, Seki et al., 2010, Das et al., 2008)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP1227

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult antennal lobe local neuron | subset

Comment: 18 per antennal lobe

(Acebes et al., 2011, Das et al., 2008)

GABAergic local interneuron of the adult antennal lobe | subset

(Das et al., 2008)

cholinergic local interneuron of the adult antennal lobe | subset

(Das et al., 2008)

adult antennal lobe local neuron lLN1

(Tanaka et al., 2012)

antennal lobe hub | restricted

(Tanaka et al., 2012)

Additional Information

Statement

Reference

Drives expression in a subset of local interneurons of adult antennal lobe, including type A local interneurons, and GABAergic and cholinergic local interneurons of the adult antennal lobe.

(FlyBase Curators, 2017)

ScerGAL4^NP1227 drives expression in around 18 type A local interneurons. Their cell bodies cluster in the dorso-lateral region of the antennal lobe. Projections are detected to all antennal lobe glomeruli and the antennal lobe hub.

(Tanaka et al., 2012)

ScerGAL4^NP1227 is expressed in 18 local neurons of the antennal lobe.

(Acebes et al., 2011)

The approximately 20 somata of cells expressing ScerGAL4^NP1227 are found in a lateral cell body cluster of the antennal lobe.

(Das et al., 2008)

Marker for

Reflects expression of

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Tanaka et al., 2012, Acebes et al., 2011, Das et al., 2008)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

99271

P{hsFLP}22, y¹ w^*; P{GawB}NP1227/CyO

Kyoto

103945

y^* w^*; P{GawB}NP1227 / CyO, P{UAS-lacZ.UW14}UW14

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

1227

(Olson et al., 2019)

GAL4-LN1

(Seki et al., 2010, Okada et al., 2009, Das et al., 2008)

GAL4-LN1s

(Das et al., 2008)

GAL4-NP1227

(Seki et al., 2010)

Gal4-LN1

(Singh et al., 2013, Das et al., 2010)

LN1-GAL4

(Fulterer et al., 2018, Melnattur et al., 2013, Sudhakaran et al., 2012)

LN1-Gal4

(Mullin et al., 2015, Sudhakaran et al., 2014, Acebes et al., 2011)

LN1-gal4

(Carreira-Rosario et al., 2016)

LN1.GAL4

(Lieber et al., 2011)

LN1^NP1227-GAL4

(Fulterer et al., 2018)

LN1Gal4

(Kanellopoulos et al., 2020, McCann et al., 2011)

NP1227

(Scholz-Kornehl and Schwärzel, 2016, Tanaka et al., 2012, Acebes et al., 2011, Thum et al., 2011, Okada et al., 2009, Das et al., 2008)

NP1227-Gal4

(Golovin et al., 2021, Kanellopoulos et al., 2020, Martelli et al., 2017, Tabuchi et al., 2015, Scheunemann et al., 2012)

Np1227-GAL4

(Rybak et al., 2016)

P{GawB}LN1^NP1227

P{GawB}NP1227

(Scheunemann et al., 2018, FlyBase, 1992-)

P{GawB}NP1227-5-1

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (28)

Report Sections

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