A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.
ScerGAL4Mmp2-NP0509 drives expression in around 9 laterally positioned adult multiglomerular antennal lobe projection neurons innervating all antennal lobe glomeruli and the antennal lobe hub.
Mmp2 reporter is expressed in the air sac primordium in third instar larvae and becomes progressively more prominent at the distal end of the air sac and in the tip cells.
Only weak expression of Mmp2 reporter is observed at late pupal stages. Expression is significantly elevated at the early adult stage (0-4 hr after eclosion) and returns to the basal level three days after eclosion. Mmp2 appears to be transiently upregulated in epithelial cells during 24 hours after eclosion.
Location 2R:5195311-5195765 determined by FlyBase alignment of dbGSS accession AG218438 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.