FlyBase Insertion Report: Dmel\P{PTT-GA}Jupiter[G00147]

General Information

Symbol

Dmel\P{PTT-GA}Jupiter^G00147

Species

D. melanogaster

Name

FlyBase ID

FBti0027868

Feature type

transposable_element_insertion_site

Also Known As

G147, Jupiter-GFP, G147-GFP, GFP-Jupiter, 17238-GFP, Jupiter::GFP

Description

Inserted Element

P{PTT-GA}

LINE ID

Affected gene(s)

Avic\GFP, Jupiter

Viability / fertility

fertile, viable

Associated allele(s)

Avic\GFP^{Jupiter-G00147}, Jupiter^G00147

Stock availability

4 publicly available

Genomic Location

Chromosomal Location

3R ( 86E12 )

Sequence Location

3R:11,604,922..11,604,922 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PTT-GA,GB,GC}.FT

Description

Additional lines created; use of a high-throughput embryo sorter allowed screening of very large numbers of animals for GFP-expressing protein trap insertions.

(Buszczak et al., 2007)

A set of transgenic insertion stocks derived by TE mobilization using the protein-trap constructs P{PTT-GA}, P{PTT-GB}, and P{PTT-GC}. The constructs carry a w^+mC mini-white visible marker and an Avic\GFP vital fluorescent protein-trap marker. In each of the three constructs, the splice acceptor and splice donor consensus sequences are in a different reading frame relative to the Avic\GFP sequence. For a successful GFP-positive insertion into an intron of a protein-coding gene, translation results in a fusion of the GFP to both the amino- and carboxyl-terminal parts of the trapped protein.

(Morin et al., 2001)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:11,604,922..11,604,922 [-]

(Morin et al., 2001)

Orientation

(Morin et al., 2001)

Cytological location (computed by FlyBase)

86E12 (inferred by FlyBase from sequence location)

Cytological location (reported)

86E16 (reported as inferred from sequence location)

(Cooley et al., 2003.5)

Insertion into Natural transposon

Comments concerning location

Insertion reported at coordinate 81655 on AE003692.1.

(Morin et al., 2001)

Sequence Data

Flanking sequence

Inserted Element

Construct

P{PTT-GA}

Size (Kb)

5.956

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Avic\GFP^{Jupiter-G00147}

Phenotypic Class Phenotype Manifest in Detailed Description

Jupiter^G00147

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 2

organism

• microtubule

(Telley et al., 2012)

third instar larval stage

cortical zone of lymph gland primary lobe

(Jung et al., 2005)

medullary zone of lymph gland primary lobe

(Jung et al., 2005)

spermatogenesis

cyst cell of testis

(Texada et al., 2008)

Additional Information

Statement

Reference

AvicGFP^{Jupiter-G00147} is localized to cortical and medullary zones of the lymph gland.

(Jung et al., 2005)

Marker for

cyst cell of testis

(Texada et al., 2008)

microtubule

(Telley et al., 2012)

Reflects expression of

Jupiter

(Telley et al., 2012, Texada et al., 2008, Jung et al., 2005, Cooley et al., 2003.5, Morin et al., 2001)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (4)

Bloomington

6836

w¹¹¹⁸; P{PTT-GA}Jupiter^G00147

54578

w^*; MCPH1^d2/CyO, P{ActGFP}JMR1; P{PTT-GA}Jupiter^G00147

99905

w^*; P{His2Av-mRFP1}III.1, P{PTT-GA}Jupiter^G00147/TM3, Ser¹

Kyoto

110606

w^* P{PTT-GA}Jupiter^G00147

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

17238-GFP

(Stumpff et al., 2005, Stumpff et al., 2004)

G0147

(Texada et al., 2008)

G147

(Stettler et al., 2015, Li et al., 2010, Chabu and Doe, 2008, Wen et al., 2008, Karpova et al., 2006, Jung et al., 2005, Pandey et al., 2005, Morin et al., 2001)

G147-GFP

(Smith and Prehoda, 2011, Siegrist and Doe, 2006, Siller et al., 2005)

GFP-G147

(Rusan and Peifer, 2007)

GFP-Jupiter

(Baskar et al., 2019, Takeda et al., 2018, Emre et al., 2011)

GFP::Jupiter

(Chabu and Doe, 2008)

Jupiter-GFP

(Planelles-Herrero et al., 2022, Villars et al., 2022, Zajac and Horne-Badovinac, 2022, Chen et al., 2019, Althoff et al., 2012, Yao et al., 2012, Sakurai et al., 2011)

Jupiter::GFP

(Daeden et al., 2023, Ducuing and Vincent, 2016)

P{PTT-GA}CG31363^G00147

(Cooley et al., 2003.5, FlyBase, 1992-)

P{PTT-GA}Jupiter ^G147

(Rolls et al., 2007)

P{PTT-GA}Jupiter^G00147

(Telley et al., 2012, FlyBase, 1992-)

P{PTT-GB}CG31363^G00147

(FlyBase, 1992-)

P{PTT-GB}CG31363^G147

(FlyBase, 1992-)

P{w[+mC]=PTT-GA}Jupiter^G001477

(Schittenhelm et al., 2007)

jupiter-GFP

(Bossing et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (36)

Report Sections

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