FlyBase Insertion Report: Dmel\P{GAL4}kra[krasavietz]

General Information

Symbol

Dmel\P{GAL4}kra^krasavietz

Species

D. melanogaster

Name

FlyBase ID

FBti0027494

Feature type

transposable_element_insertion_site

Also Known As

krasavietz-GAL4, c150-gal4

Description

Inserted Element

P{GAL4}

LINE ID

Affected gene(s)

kra, Scer\GAL4

Viability / fertility

Associated allele(s)

kra^krasavietz, Scer\GAL4^{kra-krasavietz}

Stock availability

None publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GAL4}

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

kra

(Dubnau et al., 2003)

Scer\GAL4

(Boto et al., 2014, Acebes et al., 2012, Acebes et al., 2011, Kazama et al., 2011, Lieber et al., 2011, Thum et al., 2011, Chou et al., 2010, Seki et al., 2010, Krashes et al., 2009, Das et al., 2008, Shang et al., 2007, Dubnau et al., 2003, Dubnau et al., 2003)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

kra^krasavietz

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^{kra-krasavietz}

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

intrinsic neuron of embryonic/larval antennal lobe

(Thum et al., 2011)

adult stage

central nervous system

(Dubnau et al., 2003)

adult antennal lobe local neuron

(Chou et al., 2010)

adult antennal lobe local neuron | subset

Comment: 6-8 per antennal lobe

(Acebes et al., 2011, Das et al., 2008, Shang et al., 2007)

Kenyon cell | subset

(Chou et al., 2010, Shang et al., 2007)

GABAergic neuron | subset

(Shang et al., 2007)

cholinergic neuron | subset

(Shang et al., 2007)

GABAergic local interneuron of the adult antennal lobe | subset

Comment: 12 of 16 labeled neurons

(Chou et al., 2010)

cholinergic local interneuron of the adult antennal lobe

Comment: 6-8 per antennal lobe

(Acebes et al., 2011)

adult antennal lobe projection neuron

(Acebes et al., 2011)

lobe system of mushroom body

(Acebes et al., 2011)

mushroom body spur

(Acebes et al., 2011)

dopaminergic PPL1 neuron | subset

(Acebes et al., 2011)

adult mushroom body

(Dubnau et al., 2003)

mushroom body extrinsic neuron | subset

(Cervantes-Sandoval et al., 2016)

mushroom body pedunculus-medial lobe arborizing neuron 1

(Zhang et al., 2022)

mushroom body medial-vertical lobe arborizing neuron 1

(Zhang et al., 2022)

mushroom body vertical lobe arborizing neuron 1

(Zhang et al., 2022)

Additional Information

Statement

Reference

Drives expression in both GABAergic and cholinergic local interneurons. Drives expression in dopaminergic PPL1 neurons.

(FlyBase Curators, 2017)

ScerGAL4^{kra-krasavietz} drives expression in neurons that densely innervate the larval antennal lobes (AL). The cell bodies of labeled neurons are found in two clusters, one ventrolateral and the other one dorsolateral to the AL.

(Thum et al., 2011)

ScerGAL4^{exba-krasavietz} is expressed in 6-8 local neurons of the antennal lobe, the majority of these being cholinergic. It is also expressed in antennal lobe projection neurons that innervate the mushroom body lobes and heel and a subset of the dopaminergic PPL1 neurons.

(Acebes et al., 2011)

Expression of ScerGAL4^{exba-krasavietz} is seen in cell bodies found in the local interneuron cluster located lateral and dorsolateral to the antennal lobe. Most labelled cells are ipsilaterally projecting interneurons.

(Chou et al., 2010)

ScerGAL4^{exba-krasavietz} drives expression in mostly GABAergic antennal lobe local interneurons, and in a subset of mushroom body neurons.

(Chou et al., 2010)

ScerGAL4^{exba-krasavietz} expresses in about a dozen antennal lobe local interneurons.

(Shang et al., 2007)

This GAL4 transgene inserted in the kra gene drives expression in the central nervous system with enriched expression in the mushroom bodies.

(Dubnau et al., 2003)

Marker for

Reflects expression of

kra

(Zhang et al., 2022, Cervantes-Sandoval et al., 2016, Thum et al., 2011, Chou et al., 2010, Das et al., 2008, Shang et al., 2007, Dubnau et al., 2003)

Reporter construct used in assay

Ecol\lacZ^UAS.cUa

(Chou et al., 2010)

Mmus\Cd8a^UAS.cLa.EGFP

(Acebes et al., 2011, Chou et al., 2010, Das et al., 2008, Shang et al., 2007)

Syt1^UAS.Tag:HA

(Chou et al., 2010)

Avic\GFP^{UAS.Tag:NLS(SV40-largeT)}

(Acebes et al., 2011)

Avic\GFP^{Cameleon2.1.UAS}

(Thum et al., 2011)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

C150

(Boto et al., 2014)

GAL4-Krasavietz

(Seki et al., 2010)

Kra-GAL4

(Das et al., 2010)

Kra.GAL4

(Lieber et al., 2011)

Kras-Gal4

(Gong et al., 2013)

Krasavietz-GAL4

(Thum et al., 2011, Das et al., 2008)

Krasavietz-Gal4

(Wu et al., 2019)

Line 9

(Chou et al., 2010)

P{GAL4}eIF-5C^krasavietz

(Dubnau et al., 2003, FlyBase, 1992-)

P{GAL4}exba^krasavietz

(FlyBase, 1992-)

P{GAL4}kra^krasavietz

c150-GAL4

(Phan et al., 2019, Boto et al., 2014)

c150-Gal4

(Zhang et al., 2022)

c150-gal4

(Cervantes-Sandoval et al., 2016, Berry et al., 2015)

krasavietz

(Krashes et al., 2009)

krasavietz-GAL4

(Brown et al., 2019, Chen et al., 2019, Liou et al., 2018, Melnattur et al., 2013, Shang et al., 2007)

krasavietz-Gal4

(Kuo et al., 2015, Acebes et al., 2012, Acebes et al., 2011, Kazama et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (28)

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