Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
l(2)k08901
Location 2R:12967569-12967570 determined by FlyBase alignment of dbGSS accession AQ034114 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
Two insertions present, 30kb apart, or one insertion associated with a 30kb deletion.
FlyBase curator comment: The "k08901" insertion line contains a deletion (Df(2R)k08901) (see FBrf0217415). It is not clear how many insertions are present in this line: although the 5' (AQ034114) and 3' (AQ025874) flanking sequences map 30kb apart, consistent with the presence of the deletion, the reported location of the P-element insertion site in the two flanking sequences appear to be incompatible if only one insertion is present. It is possible that there is one insertion in the line and that there is an error in the reported sequence data, or there may be two insertions in the line. Until more information is known, a separate insertion record exists in FlyBase for each flanking sequence: P{lacW}k08901b (FBti0025322) corresponds to the flanking sequence reported in AQ034114, P{lacW}l(2)k01209k08901a (FBti0006619) corresponds to the flanking sequence reported in AQ025874.