FlyBase Insertion Report: Dmel\P{GawB}GH298

General Information

Symbol

Dmel\P{GawB}GH298

Species

D. melanogaster

Name

FlyBase ID

FBti0016782

Feature type

transposable_element_insertion_site

Also Known As

GH298-Gal4, GH298, GAL4-GH298, GH298Gal4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^GH298

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 99F )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

99F (in situ hybridization reported)

(Kadowaki, 2011.10.7)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(FlyBase, 1992-)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Li et al., 2013, Acebes et al., 2012, Volders et al., 2012, Acebes et al., 2011, Kazama et al., 2011, Thum et al., 2011, Carlsson et al., 2010, Chou et al., 2010, Jansen et al., 2009, Lai et al., 2008, Shang et al., 2007, Zhu et al., 2006, Wilson and Laurent, 2005, Hummel and Zipursky, 2004, Wilson et al., 2004, Hummel et al., 2003, Stocker et al., 1997)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^GH298

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

intrinsic neuron of embryonic/larval antennal lobe

(Thum et al., 2011)

pupal stage

antennal lobe

(Stocker et al., 1997)

adult stage

gnathal ganglion

(Stocker et al., 1997)

antenna

(Hummel and Zipursky, 2004)

olfactory receptor neuron of antenna | subset

(Hummel and Zipursky, 2004)

antennal lobe glomerulus V

(Hummel and Zipursky, 2004)

olfactory receptor neuron | subset

(Hummel and Zipursky, 2004)

GABAergic local interneuron of the adult antennal lobe

(Acebes et al., 2011, Wilson and Laurent, 2005)

adult antennal lobe projection neuron | subset

(Chou et al., 2010)

adult olfactory receptor neuron | subset

(Chou et al., 2010)

GABAergic local interneuron of the adult antennal lobe | subset

Comment: 24 of 28 labeled neurons

(Chou et al., 2010, Lai et al., 2008)

cholinergic local interneuron of the adult antennal lobe | subset

Comment: 1 of 28 labeled neurons

(Chou et al., 2010)

adult antennal lobe local neuron

(Scheunemann et al., 2019, Chou et al., 2010)

GABAergic neuron | subset

(Shang et al., 2007)

cholinergic neuron | subset

(Shang et al., 2007)

adult antennal lobe projection neuron lPN | subset

(Lai et al., 2008)

adult antennal lobe local neuron | subset

Comment: 30-32 per antennal lobe

(Acebes et al., 2011)

adult antennal lobe

(Stocker et al., 1997)

adult gnathal ganglion serotonergic projection neuron

(Scheunemann et al., 2019)

adult neuron | subset of adult brain

(Scheunemann et al., 2019)

Additional Information

Statement

Reference

Drives expression in mostly GABAergic antennal lobe local interneurons, and in a subset of olfactory projection neurons and olfactory receptor neurons.

(FlyBase Curators, 2017)

ScerGAL4^GH298 drives expression in neurons with cell bodies located lateral to the antennal lobes (AL). Some of the labeled neurons innervate the AL.

(Thum et al., 2011)

ScerGAL4^GH298 is expressed in 30-32 local neurons of the antennal lobe, with almost all being GABAergic.

(Acebes et al., 2011)

Expression of ScerGAL4^GH298 is seen in cell bodies found in the local interneuron cluster located lateral and dorsolateral to the antennal lobe. Most labelled cells are ipsilaterally projecting interneurons.

(Chou et al., 2010)

ScerGAL4^H24 drives expression in mostly GABAergic antennal lobe local interneurons, and in a subset of olfactory projection neurons and olfactory receptor neurons.

(Chou et al., 2010)

There are an average 21 ScerGAL4^GH298-positive lPN cells.

(Lai et al., 2008)

An estimated 81% cells expressing ScerGAL4^GH298 are GABAergic.

(Shang et al., 2007)

The neurites of the AL LNs collectively fill the antennal lobes. Expression of ScerGAL4^GH298 generally colocalizes with GABA-immunoreactivity, though approximately one-fifth of the GFP positive cells are not GABA-immunopositive.

(Wilson and Laurent, 2005)

In adult brain, expression observed in about 20 neurons with arborization in the antennal lobe (AL), most with cell bodies lateral to the AL; pattern fits the shape of 'LocI' (FBrf0051437); expression observed in a subset of neurons in the subesophageal ganglion. No expression observed in the AL in third instar larvae or early pupae; starts to develop at 48h APF.

(Stocker et al., 1997)

Marker for

antennal lobe glomerulus V

(Hummel and Zipursky, 2004)

Reflects expression of

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Chou et al., 2010, Lai et al., 2008, Shang et al., 2007, Wilson and Laurent, 2005)

Ecol\lacZ^UAS.cUa

(Chou et al., 2010)

Syt1^UAS.Tag:HA

(Chou et al., 2010)

Avic\GFP^{UAS.Tag:NLS(SV40-largeT)}

(Acebes et al., 2011)

Avic\GFP^{Cameleon2.1.UAS}

(Thum et al., 2011)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

37294

w^*; P{GawB}GH298

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

GAL4-GH298

(Lai et al., 2008)

GH298

(Tsai et al., 2018, Scholz-Kornehl and Schwärzel, 2016, Li et al., 2013, Acebes et al., 2012, Volders et al., 2012, Thum et al., 2011, Jansen et al., 2009)

GH298-GAL4

(Rozenfeld et al., 2021, Brown et al., 2019, Minocha et al., 2017, Tanaka et al., 2012, Carlsson et al., 2010, Shang et al., 2007)

GH298-Gal4

(Hu et al., 2019, Scheunemann et al., 2019, Wu et al., 2019, Scheunemann et al., 2018, Casas-Tintó et al., 2017, Nagel and Wilson, 2016, Gong et al., 2013, Scheunemann et al., 2012, Acebes et al., 2011, Kazama et al., 2011, Carlsson et al., 2010, Ignell et al., 2009, Root et al., 2008, Zhu et al., 2006, Wilson and Laurent, 2005, Hummel and Zipursky, 2004, Hummel et al., 2003)

GH298GAL4

(Hamid et al., 2021)

GH298Gal4

(Carlsson et al., 2010)

Gal4-GH298

(Zhu et al., 2006)

Line 4

(Chou et al., 2010)

P{GAL4}GH298

(Stocker and Heimbeck, 2011.9.21, Stocker et al., 1997, FlyBase, 1992-)

P{GawB}GH298

(Kadowaki, 2011.10.7)

Name Synonyms

Secondary FlyBase IDs

References (38)

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