Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
A set of X chromosome mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or semi-lethal phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
There is a 609 bp flanking sequence record in GenBank (AJ426942) that is annotated as having been isolated by 5' and 3' inverse PCR with positions 93-100 being the 8 bp target duplication after the insertion. There are 10 consecutive n's in the sequence at positions 101-110. The 609 bp sequence aligns in two segments. Segment 25-100 aligns to X:15733653-15733728 [+] and segment 111-609 aligns to X:15733921-15734419 [+]. This could mean that there is an insertion associated with a deletion, or rather the 10 n's just after the target site duplication may indicate that the inverse PCR did not recover both vector-flank junctions and that the 10 n's indicates a gap in the sequence. Based on this interpretation of the GenBank record, the sequence location range is X:15733728..15733921 .
P{lacW}l(1)G0164
