FB2024_03 , released June 25, 2024
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Levis, R. (2018.11.27). Sequence location of insertion P{lacW}l(1)G0164[G0164]. 
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FBrf0240891
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Personal communication to FlyBase
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The sequence location in FlyBase for P-element insertion P{lacW}l(1)G0164G0164 (FBti0013194) is given as a range  X:15 ,733,960..15,734,419 + (release 6) and the Affected Gene entry is l(1)G0164  (FB2018_05 release).  There is a 609 bp flanking sequence record in GenBank (AJ426942) that is annotated as having been isolated by 5' and 3' inverse PCR with positions 93-100 being the 8 bp target duplication after the insertion.  There are 10 consecutive n's in the sequence at positions 101-110.  The 609 bp sequence aligns in two segments.  Segment 25-100 aligns to  X:15733653-15733728+ and segment 111-609 aligns to  X:15733921-15734419+.  This could mean that there is an insertion associated with a deletion, but my suspicion is that the 10 n's just after the target site duplication may indicate that the inverse PCR did not recover both vector-flank junctions and that the 10 n's indicates a gap in the sequence.  Based on this interpretation of the GenBank record, the sequence location range is  X:15733728..15733921 .  Both ends of this range are within the CG12379 gene and therefore this is the most likely gene that is affected by this insertion.
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    English
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