FlyBase Insertion Report: Dmel\P{PZ}slmb[00295]

General Information

Symbol

Dmel\P{PZ}slmb⁰⁰²⁹⁵

Species

D. melanogaster

Name

FlyBase ID

FBti0010370

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)00295

Affected gene(s)

Ecol\lacZ, slmb

Viability / fertility

Associated allele(s)

Ecol\lacZ^slmb-00295, slmb⁰⁰²⁹⁵

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

3R ( 93C1 )

Sequence Location

3R:21,127,176..21,127,176

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:21,127,176..21,127,176

(Jiang and Struhl, 1998)

Orientation

Cytological location (computed by FlyBase)

93C1 (inferred by FlyBase from sequence location)

Cytological location (reported)

93B10-93B11 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

The insertion site was mapped to 182 bases upstream of the slmb ATG.

(Jiang and Struhl, 1998)

Sequence Data

Flanking sequence

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Muzzopappa and Wappner, 2005, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

slmb

(Zhang et al., 2015, Roberts et al., 2012, Kuo et al., 2006, Jiang and Struhl, 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^slmb-00295

Phenotypic Class Phenotype Manifest in Detailed Description

slmb⁰⁰²⁹⁵

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval hindgut

(Meister and Braun, 1995.10)

adult stage

third segment of antenna

(Vosshall, 1998.8.10)

oogenesis

nurse cell