FlyBase Insertion Report: Dmel\P{lacW}Dad[j1E4]

General Information

Symbol

Dmel\P{lacW}Dad^j1E4

Species

D. melanogaster

Name

FlyBase ID

FBti0009904

Feature type

transposable_element_insertion_site

Also Known As

dad-lacZ, Dad^j1E4-lacZ

Description

Inserted Element

P{lacW}

LINE ID

l(3)j1E4

Affected gene(s)

Dad, Ecol\lacZ

Viability / fertility

Associated allele(s)

Dad^j1E4, Ecol\lacZ^Dad-j1E4

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

3R ( 89E11 )

Sequence Location

3R:17,065,723..17,065,723 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:17,065,723..17,065,723 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

89E11 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 3R:12891446..12891454 .

Sequence Data

Flanking sequence

AQ026297

(FlyBase, 1992-)

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Dad

(Toraason et al., 2024, Park et al., 2022, Ogiso et al., 2011, BDGP Project Members, 1994-1999)

Ecol\lacZ

(Eliazer et al., 2011, Oh and Irvine, 2011, Zhao et al., 2008, Mohit et al., 2006, Tsuneizumi et al., 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Dad^j1E4

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^Dad-j1E4

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Tsuneizumi et al., 1997)

larval stage

larval ventral nerve cord | restricted

(Vuilleumier et al., 2019)

third instar larval stage

wing disc | restricted

(Tsuneizumi et al., 1997)

larval ventral nerve cord

(Vuilleumier et al., 2019)

adult stage

adult midgut

(Guo et al., 2013)

adult midgut region R3a

(Guo et al., 2013)

adult midgut region R3b

(Guo et al., 2013)

adult s-LNv neuron

(Beckwith et al., 2013)

DN1 neuron | subset

(Beckwith et al., 2013)

Additional Information

Statement

Reference

The Dad reporter is expressed in ~43% of pMad-positive nuclei in the ventral nerve cord.

(Vuilleumier et al., 2019)

Dad reporter is expressed in 414 +/- 26 nuclei of the larval ventral nerved cord. This is about 43% of the pMad-positive nuclei in the ventral nerve cord.

(Vuilleumier et al., 2019)

EcollacZ^Dad-j1E4 expression is observed in the anterior and posterior adult midgut, and is expressed at high levels in and adjacent to the copper cell region (CCR, adult midgut anterior regions R3a and R3b). EcollacZ^Dad-j1E4 is a marker for BMP signalling.

(Guo et al., 2013)

EcollacZ^Dad-j1E4 expression is absent from the LNd neurons, clearly present in the small and large s-LNv neurons , and is also present in a few DN1 neurons.

(Beckwith et al., 2013)

Expression pattern of this reporter construct during embryonic and imaginal development resembles that of dpp.

(Tsuneizumi et al., 1997)

Marker for

Reflects expression of

Dad

(Vuilleumier et al., 2019, Beckwith et al., 2013, Guo et al., 2013)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)j1E4

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

10305

y¹ w¹¹¹⁸; P{lacW}Dad^j1E4/TM3, Sb¹

Kyoto

111046

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B P{lacW}Dad^j1E4/TM6B, P{Car20y}TPN1, Tb¹

External Crossreferences and Linkouts ( 0 )

Comments

Location 3R:12891445-12891446 confirmed by FlyBase alignment of dbGSS accession AQ026297 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)

insertion associated host repeat

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

Dad-LacZ

(West et al., 2015, Eliazer et al., 2011)

Dad-lacZ

(Zappia et al., 2020, Guo et al., 2013, Zhao et al., 2008)

Dad^j1E4

(Ogiso et al., 2011)

Dad^j1E4-LacZ

(Roy et al., 2014)

Dad^j1E4-lacZ

(Mohit et al., 2006)

P{LacW}Dad^J1E4

(West et al., 2015)

P{lacW}Dad^j1E4

(Shidlovskii et al., 2021, FlyBase, 2005, FlyBase, 1992-)

P{lacW}l(3)j1E4^j1E4

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{lacZ}l(3)1E4

(Tsuneizumi et al., 1997, FlyBase, 1992-)

dad-lacZ

(Oh and Irvine, 2011, Roy et al., 2011, Zhao et al., 2008, BDGP Project Members, 1994-1999)

dad^j1E4

(Roy et al., 2011)

dad^j1E4-lacZ

(Vuilleumier et al., 2019)

dadLacZ

(Beckwith et al., 2013)

Name Synonyms

Secondary FlyBase IDs

FBti0009616

References (46)

Report Sections

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