FlyBase Insertion Report: Dmel\P{PZ}lmgA[03424]

General Information

Symbol

Dmel\P{PZ}lmgA⁰³⁴²⁴

Species

D. melanogaster

Name

FlyBase ID

FBti0009120

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)03424

Affected gene(s)

Ecol\lacZ, lmgA, lmgB, Orct

Viability / fertility

Associated allele(s)

Ecol\lacZ^lmg-03424, lmgA⁰³⁴²⁴, lmgB⁰³⁴²⁴

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 29D5 )

Sequence Location

2L:8,528,450..8,528,450 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:8,528,450..8,528,450 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

29D5 (inferred by FlyBase from sequence location)

Cytological location (reported)

29D4-29D5 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025610

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Russell et al., 1998, Vosshall, 1998.8.10, Taylor and Shirras, 1995)

lmgA

(Nagy et al., 2012, Kuo et al., 2006, Watts et al., 2003, Shirras, 2000.1.5, Spradling et al., 1999, Taylor et al., 1997, FlyBase, 1996-, Taylor and Shirras, 1995, BDGP Project Members, 1994-1999)

lmgB

(FlyBase, 1996-)

Orct

(Taylor and Shirras, 1995)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^lmg-03424

Phenotypic Class Phenotype Manifest in Detailed Description

lmgA⁰³⁴²⁴

Phenotypic Class Phenotype Manifest in Detailed Description

lmgB⁰³⁴²⁴

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

stomodeum

(Jackle and Janning, 1998.8.10)

presumptive embryonic/larval peripheral nervous system | restricted

(Jackle and Janning, 1998.8.10)

organism | segmentally repeated

(Jackle and Janning, 1998.8.10)

presumptive embryonic/larval central nervous system | restricted

(Jackle and Janning, 1998.8.10)

mesoderm

(Jackle and Janning, 1998.8.10)

embryonic/larval brain

(Jackle and Janning, 1998.8.10)

wandering third instar larval stage

larval hindgut imaginal ring

(Meister and Braun, 1995.10)

Additional Information

Statement

Reference

In early embryo, expressed in 3 stripes; weak expression in mesoderm. Later expression in stomodeum and neural derivatives.

(Jackle and Janning, 1998.8.10)

Marker for

Reflects expression of

Orct

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)03424

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11330

P{PZ}lmgA⁰³⁴²⁴ lmgB⁰³⁴²⁴ cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2L:8528450-8528451 confirmed by FlyBase alignment of dbGSS accession AQ025610 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P{PZ}Orct⁰³⁴²⁴

(Taylor and Shirras, 1995, FlyBase, 1992-)

P{PZ}l(2)03424⁰³⁴²⁴

(FlyBase, 1992-)

P{PZ}lmg⁰³⁴²⁴

(FlyBase, 2005, Taylor and Shirras, 1995, FlyBase, 1992-)

P{PZ}lmgA⁰³⁴²⁴

P{ry^+t7.2=PZ}l(2)03424⁰³⁴²⁴

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000318
FBti0003605

References (19)

Report Sections

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