Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w+mC mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
EP(3)0359
Location 3R:6182230-6182231 confirmed by FlyBase alignment of dbGSS accession AQ025068 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
The insertion sites of P{Mae-GMR.E}BruceGMREP-86a-3, P{Mae-GMR.E}BruceGMREP-86a-4, P{Mae-GMR.E}BruceGMREP-86a-5, P{EP}stich1EP359, P{EP}jumuEP739, P{PZ}jumu06142 and P{lacW}l(3)j8B6j8B6 are within 6 base pairs of each other.
insertion of mobile activating element