FB2024_03 , released June 25, 2024
Insertion: Dmel\P{PZ}vn10567
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General Information
Symbol
Dmel\P{PZ}vn10567
Species
D. melanogaster
Name
FlyBase ID
FBti0005605
Feature type
transposable_element_insertion_site
Also Known As
vn-lacZ, vein-lacZ
Description
Inserted Element
P{PZ}
LINE ID

l(3)10567

Affected gene(s)
Ecol\lacZ, vn
Viability / fertility
Associated allele(s)
Ecol\lacZvn-10567, vn10567
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
3L ( 64F2 )
Sequence Location
3L:5,844,269..5,844,269 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:5,844,269..5,844,269 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)
64F2 (inferred by FlyBase from sequence location)
Cytological location (reported)
64F1-64F3 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AQ034078
(FlyBase, 1992-)
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage
imaginal disc
(Meister and Braun, 1995.10)
Malpighian tubule | restricted
(Meister and Braun, 1995.10)
embryonic/larval hindgut
(Meister and Braun, 1995.10)
circular visceral muscle fiber
(Jiang and Edgar, 2009)
embryonic/larval midgut longitudinal visceral muscle cell
(Jiang and Edgar, 2009)
pupal stage P5(ii) -- pupal stage P6
antennal disc | restricted

Comment: reference states 18-20 hr APF

(Sen et al., 2004)
adult stage
adult visceral muscle cell
(Zhou et al., 2013)
circular visceral muscle fiber
(Xu et al., 2011)
longitudinal visceral muscle fiber
(Xu et al., 2011)
Additional Information
Statement
Reference

Reporter expression was examined in the adult posterior midgut. A high level of expression was observed in the inner circular muscles and a low level was observed in the outer longitudinal muscles but none was detected in any epithelial cells.

(Xu et al., 2011)

EcollacZvn-10567 expression is stronger in the inner layer of circular visceral muscle than in the outer larval of longitudinal visceral muscle in the larval midgut.

(Jiang and Edgar, 2009)

Expression is observed in the region of the pupal antenna that is occupied mainly by coeloconic sensilla. Expression is not observed in glial cells.

(Sen et al., 2004)
 
Marker for
Reflects expression of
vn
(Zhou et al., 2013, Jiang and Edgar, 2009, Sen et al., 2004)
Reporter construct used in assay
Data on Genetic Line
Line ID

l(3)10567

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 3L:5818216-5818217 confirmed by FlyBase alignment of dbGSS accession AQ034078 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)
Synonyms and Secondary IDs (11)
Reported As
Symbol Synonym
P{PZ}l(3)1056710567
(FlyBase, 1992-)
P{PZ}vn 10567
(Kiger et al., 2000)
P{PZ}vn10567
(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{ry+t7.2=PZ}l(3)1056710567
(FlyBase, 1992-)
vein-lacZ
(Buchon et al., 2010, Sen et al., 2004)
vein-lacZ.nls
(Zhou et al., 2013)
vn-lacZ
(Tang et al., 2021, Zhou et al., 2013, Xu et al., 2011)
vn-lacZ(P1749)
(Jiang et al., 2011)
vn-lacZ10567
(Takemura and Adachi-Yamada, 2011)
vnlacZ
(Jiang and Edgar, 2009)
vnlacZP1749
(Jiang and Edgar, 2009)
Name Synonyms
Secondary FlyBase IDs
  • FBti0000661
References (26)