FlyBase Insertion Report: Dmel\P{PZ}Vha68-2[01510]

General Information

Symbol

Dmel\P{PZ}Vha68-2⁰¹⁵¹⁰

Species

D. melanogaster

Name

FlyBase ID

FBti0005196

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)01510

Affected gene(s)

Ecol\lacZ, Vha68-2

Viability / fertility

Associated allele(s)

Ecol\lacZ^{Vha68-2-01510}, Vha68-2⁰¹⁵¹⁰

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 34A3 )

Sequence Location

2L:12,975,059..12,975,059 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:12,975,059..12,975,059 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

34A3 (inferred by FlyBase from sequence location)

Cytological location (reported)

34A1-34A2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ073265

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Vha68-2

(Vaccari et al., 2010, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^{Vha68-2-01510}

Phenotypic Class Phenotype Manifest in Detailed Description

Vha68-2⁰¹⁵¹⁰

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval peripheral nervous system

(Jackle and Janning, 1998.8.10)

presumptive embryonic/larval central nervous system

(Jackle and Janning, 1998.8.10)

embryonic/larval hindgut

(Jackle and Janning, 1998.8.10)

embryonic/larval anterior midgut

(Jackle and Janning, 1998.8.10)

embryonic/larval posterior midgut

(Jackle and Janning, 1998.8.10)

wandering third instar larval stage

embryonic/larval foregut

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

Malpighian tubule

(Meister and Braun, 1995.10)

embryonic/larval hindgut

(Meister and Braun, 1995.10)

adult stage

adult optic lobe

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In embryos, expression in PNS not certain.

(Jackle and Janning, 1998.8.10)

In wandering third instar larva, high levels of expression in midgut, hindgut, and Malpighian tubules; weak expression in foregut.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Vha68-2

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)01510

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11065

P{PZ}Vha68-2⁰¹⁵¹⁰ cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P1065

(Meister and Braun, 1995.10)

P^PZl(2)⁰¹⁵¹⁰

(Vaccari et al., 2010)

P{PZ}Vha68-2⁰¹⁵¹⁰

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}l(2)01510⁰¹⁵¹⁰

(FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)01510⁰¹⁵¹⁰

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000273

References (10)

Report Sections

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