FB2024_03 , released June 25, 2024
Insertion: Dmel\P{PZ}slbo01310
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General Information
Symbol
Dmel\P{PZ}slbo01310
Species
D. melanogaster
Name
FlyBase ID
FBti0003274
Feature type
transposable_element_insertion_site
Also Known As
slbo-lacZ, PZ1310
Description
Inserted Element
P{PZ}
LINE ID
Affected gene(s)
Ecol\lacZ, slbo
Viability / fertility
Associated allele(s)
Ecol\lacZslbo-01310, slbo01310
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2R ( 60C6 )
Sequence Location
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
60C6 (near gene of known cytology)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
L00632
(FlyBase, 1992-)
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage & oogenesis stage S10
border follicle cell
(Rorth et al., 1998)
follicle cell
(Rorth et al., 1998)
oogenesis stage S8 -- S10 & adult stage | female
border follicle cell
(Gunawan et al., 2013)
oogenesis stage S9 -- S10 & adult stage | female
border follicle cell
(Wang et al., 2007)
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis stage S9 & adult stage | female
border follicle cell
(Lucas et al., 2013)
oogenesis stage S9 -- S14
border follicle cell
(Levine et al., 2010)
oogenesis stage S10A
centripetal follicle cell
(Levine et al., 2010)
stretch follicle cell
(Levine et al., 2010)
oogenesis stage S11
centripetal follicle cell

Comment: leading edge

(Levine et al., 2010)
Additional Information
Statement
Reference

slbo reporter is first detected in border follicle cells at oogenesis stage S9. Expression is detected at stage 10A in border cells, centripally migrating follicle cells, and nurse follicle cells. Levels are high in the centripetally migrating follicle cells at oogenesis stage S10A and then decrease in stage S10B when ct levels become high. By stage S11, reporter expression persists only in the follicle cells at the leading edge of centripetal migration, cells that are also marked by ct and eya and at the posterior pole. In the border cell cluster slbo reporter is expressed in both the inner polar cells and the outer border cell rosette where ct can be seen at well.

(Levine et al., 2010)
 
Marker for
border follicle cell
(Lucas et al., 2013, Wang et al., 2007)
Reflects expression of
slbo
(Gunawan et al., 2013, Lucas et al., 2013, Rorth et al., 1998)
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (11)
References (49)